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http://hdl.handle.net/2445/126387
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DC Field | Value | Language |
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dc.contributor.author | Hernando, Henar | - |
dc.contributor.author | Islam, Abul B. M. M. K. | - |
dc.contributor.author | Rodríguez Ubreva, Javier | - |
dc.contributor.author | Forné, Ignasi | - |
dc.contributor.author | Ciudad, Laura | - |
dc.contributor.author | Imhof, Axel | - |
dc.contributor.author | Shannon-Lowe, Claire | - |
dc.contributor.author | Ballestar Tarín, Esteban | - |
dc.date.accessioned | 2018-11-23T12:28:40Z | - |
dc.date.available | 2018-11-23T12:28:40Z | - |
dc.date.issued | 2014-01-01 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126387 | - |
dc.description.abstract | Epstein-Barr virus (EBV) infects and transforms human primary B cells inducing indefinite proliferation. To investigate the potential participation of chromatin mechanisms during the EBV-mediated transformation of resting B cells we performed an analysis of global changes in histone modifications. We observed a remarkable decrease and redistribution of heterochromatin marks including H4K20me3, H3K27me3 and H3K9me3. Loss of H4K20me3 and H3K9me3 occurred at constitutive heterochromatin repeats. For H3K27me3 and H3K9me3, comparison of ChIP-seq data revealed a decrease in these marks in thousands of genes, including clusters of HOX and ZNF genes, respectively. Moreover, DNase-seq data comparison between resting and EBV-transformed B cells revealed increased endonuclease accessibility in thousands of genomic sites. We observed that both loss of H3K27me3 and increased accessibility are associated with transcriptional activation. These changes only occurred in B cells transformed with EBV and not in those stimulated to proliferate with CD40L/IL-4, despite their similarities in the cell pathways involved and proliferation rates. In fact, B cells infected with EBNA-2 deficient EBV, which have much lower proliferation rates, displayed similar decreases for heterochromatic histone marks. Our study describes a novel phenomenon related to transformation of B cells, and highlights its independence of the pure acquisition of proliferation. | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1093/nar/gkt886 | - |
dc.relation.ispartof | Nucleic Acids Research, 2014, vol. 42, num. 1, p. 249-263 | - |
dc.relation.uri | https://doi.org/10.1093/nar/gkt886 | - |
dc.rights | cc by (c) Hernando et al., 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Herpesvirus | - |
dc.subject.classification | Cèl·lules B | - |
dc.subject.other | Herpesviruses | - |
dc.subject.other | B cells | - |
dc.title | Epstein–Barr virus-mediated transformation of B cells induces global chromatin changes independent to the acquisition of proliferation | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2018-07-24T12:44:08Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/257082/EU//EPIGENESYS | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 24097438 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Publicacions de projectes de recerca finançats per la UE |
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HernandoH.pdf | 16.21 MB | Adobe PDF | View/Open |
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