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http://hdl.handle.net/2445/126429
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DC Field | Value | Language |
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dc.contributor.author | Ciruela Alférez, Francisco | - |
dc.contributor.author | Vilardaga, Jean Pierre | - |
dc.contributor.author | Fernández Dueñas, Víctor | - |
dc.date.accessioned | 2018-11-26T12:12:12Z | - |
dc.date.available | 2018-11-26T12:12:12Z | - |
dc.date.issued | 2010-08 | - |
dc.identifier.issn | 0167-7799 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126429 | - |
dc.description.abstract | Spatiotemporal characterization of protein protein interactions (PPIs) is essential in determining the molecular mechanisms of intracellular signaling processes. In this review, we discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. These technologies alone or in concert with complementary methods (SRET, BRET or BiFC, and SNAP-tag or TR-FRET) can be extremely powerful approaches for PPI visualization, even between more than two proteins. Here we provide a comprehensive update on all the biotechnological aspects, including the strengths and weaknesses, of new fluorescence- and luminescence-based methodologies, with a specific focus on their application for studying PPIs. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Current Trends | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.tibtech.2010.05.002 | - |
dc.relation.ispartof | Trends in Biotechnology, 2010, vol. 28, num. 8, p. 407-415 | - |
dc.relation.uri | https://doi.org/10.1016/j.tibtech.2010.05.002 | - |
dc.rights | (c) Elsevier Current Trends, 2010 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Proteïnes | - |
dc.subject.classification | Oligòmers | - |
dc.subject.other | Proteins | - |
dc.subject.other | Oligomers | - |
dc.title | Lighting up multiprotein complexes: lessons from GPCR oligomerization | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 578941 | - |
dc.date.updated | 2018-11-26T12:12:12Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 20542584 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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578941.pdf | 869.22 kB | Adobe PDF | View/Open |
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