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DC Field | Value | Language |
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dc.contributor.author | Fontrodona, Laura | - |
dc.contributor.author | Porta de la Riva, Montserrat | - |
dc.contributor.author | Morán, Tomás | - |
dc.contributor.author | Niu, Wei | - |
dc.contributor.author | Diaz, Mònica | - |
dc.contributor.author | Aristizábal Corrales, David | - |
dc.contributor.author | Villanueva Garatachea, Alberto | - |
dc.contributor.author | Schwartz Navarro, Simó | - |
dc.contributor.author | Reinke, Valerie | - |
dc.contributor.author | Cerón Madrigal, Julián | - |
dc.date.accessioned | 2018-11-27T09:23:29Z | - |
dc.date.available | 2018-11-27T09:23:29Z | - |
dc.date.issued | 2013-06-06 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126462 | - |
dc.description.abstract | Protein components of the spliceosome are highly conserved in eukaryotes and can influence several steps of the gene expression process. RSR-2, the Caenorhabditis elegans ortholog of the human spliceosomal protein SRm300/SRRM2, is essential for viability, in contrast to the yeast ortholog Cwc21p. We took advantage of mutants and RNA interference (RNAi) to study rsr-2 functions in C. elegans, and through genetic epistasis analysis found that rsr-2 is within the germline sex determination pathway. Intriguingly, transcriptome analyses of rsr-2(RNAi) animals did not reveal appreciable splicing defects but instead a slight global decrease in transcript levels. We further investigated this effect in transcription and observed that RSR-2 colocalizes with DNA in germline nuclei and coprecipitates with chromatin, displaying a ChIP-Seq profile similar to that obtained for the RNA Polymerase II (RNAPII). Consistent with a novel transcription function we demonstrate that the recruitment of RSR-2 to chromatin is splicing-independent and that RSR-2 interacts with RNAPII and affects RNAPII phosphorylation states. Proteomic analyses identified proteins associated with RSR-2 that are involved in different gene expression steps, including RNA metabolism and transcription with PRP-8 and PRP-19 being the strongest interacting partners. PRP-8 is a core component of the spliceosome and PRP-19 is the core component of the PRP19 complex, which interacts with RNAPII and is necessary for full transcriptional activity. Taken together, our study proposes that RSR-2 is a multifunctional protein whose role in transcription influences C. elegans development. | - |
dc.format.extent | 18 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pgen.1003543 | - |
dc.relation.ispartof | PLoS Genetics, 2013, vol. 9, num. 6, p. e1003543 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pgen.1003543 | - |
dc.rights | cc by (c) Fontrodona et al., 2013 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.classification | Cromatina | - |
dc.subject.other | Gene expression | - |
dc.subject.other | Chromatin | - |
dc.title | RSR-2, the Caenorhabditis elegans Ortholog of Human Spliceosomal Component SRm300/SRRM2, Regulates Development by Influencing the Transcriptional Machinery | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2018-07-24T12:48:17Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/206584/EU//CANCEROMICS | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23754964 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Publicacions de projectes de recerca finançats per la UE |
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File | Description | Size | Format | |
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FontrodonaL.pdf | 3.08 MB | Adobe PDF | View/Open |
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