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Title: | Imprinting at the PLAGL1 domain is contained within a 70-kb CTCF/cohesin-mediated non-allelic chromatin loop |
Author: | Iglesias Platas, Isabel Court, Franck Camprubí Sánchez, Cristina Sparago, Angela Guillaumet Adkins, Amy Martín Trujillo, Alex Riccio, Andrea Moore, Gudrun E. Monk, Dave Nicholas |
Keywords: | Regulació genètica Cromatina Genetic regulation Chromatin |
Issue Date: | 7-Jan-2013 |
Publisher: | Oxford University Press |
Abstract: | Paternal duplications of chromosome 6q24, a region that contains the imprinted PLAGL1 and HYMAI transcripts, are associated with transient neonatal diabetes mellitus. A common feature of imprinted genes is that they tend to cluster together, presumably as a result of sharing common cis-acting regulatory elements. To determine the extent of this imprinted cluster in human and mouse, we have undertaken a systematic analysis of allelic expression and DNA methylation of the genes mapping within an similar to 1.4-Mb region flanking PLAGL1/Plagl1. We confirm that all nine neighbouring genes are biallelically expressed in both species. In human we identify two novel paternally expressed PLAGL1 coding transcripts that originate from unique promoter regions. Chromatin immunoprecipitation for CTCF and the cohesin subunits RAD21 and SMC3 reveals evolutionarily conserved binding sites within unmethylated regions similar to 5 kb downstream of the PLAGL1 differentially methylated region and within the PLAGL1 3' untranslated region (UTR). Higher-order chromatin looping occurs between these regions in both expressing and non-expressing tissues, forming a non-allelic chromatin loop around the PLAGL1/Plagl1 gene. In placenta and brain tissues, we identify an additional interaction between the PLAGL1 P3/P4 promoters and the unmethylated element downstream of the PLAGL1 differentially methylated region that we propose facilitates imprinted expression of these alternative isoforms. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/nar/gks1355 |
It is part of: | Nucleic Acids Research, 2013, vol. 41, num. 4, p. 2171-2179 |
URI: | http://hdl.handle.net/2445/126472 |
Related resource: | https://doi.org/10.1093/nar/gks1355 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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Iglesias-PlatasI.pdf | 6.33 MB | Adobe PDF | View/Open |
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