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http://hdl.handle.net/2445/126517
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DC Field | Value | Language |
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dc.contributor.author | Garcia Linares, Carles | - |
dc.contributor.author | Mercadé, Jaume | - |
dc.contributor.author | Gel, Bernat | - |
dc.contributor.author | Biayna, Josep | - |
dc.contributor.author | Terribas Pérez, Ernest | - |
dc.contributor.author | Lázaro García, Conxi | - |
dc.contributor.author | Serra Arenas, Eduard | - |
dc.date.accessioned | 2018-11-28T09:13:35Z | - |
dc.date.available | 2018-11-28T09:13:35Z | - |
dc.date.issued | 2012-08-15 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126517 | - |
dc.description.abstract | The study of somatic genetic alterations in tumors contributes to the understanding and management of cancer. Genetic alterations, such us copy number or copy neutral changes, generate allelic imbalances (AIs) that can be determined using polymorphic markers. Here we report the development of a simple set of calculations for analyzing microsatellite multiplex PCR data from control-tumor pairs that allows us to obtain accurate information not only regarding the AI status of tumors, but also the percentage of tumor-infiltrating normal cells, the locus copy-number status and the mechanism involved in AI. We validated this new approach by re-analyzing a set of Neurofibromatosis type 1-associated dermal neurofibromas and comparing newly generated data with results obtained for the same tumors in a previous study using MLPA, Paralog Ratio Analysis and SNP-array techniques. Microsatellite multiplex PCR analysis (MMPA) should be particularly useful for analyzing specific regions of the genome containing tumor suppressor genes and also for determining the percentage of infiltrating normal cells within tumors allowing them to be sorted before they are analyzed by more expensive techniques. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0042682 | - |
dc.relation.ispartof | PLoS One, 2012, vol. 7, num. 8, p. e42682 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0042682 | - |
dc.rights | cc by (c) Garcia Linares et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Neurofibromatosi | - |
dc.subject.classification | Reacció en cadena de la polimerasa | - |
dc.subject.other | Neurofibromatosis | - |
dc.subject.other | Polymerase chain reaction | - |
dc.title | Applying Microsatellite Multiplex PCR Analysis (MMPA) for Determining Allele Copy-Number Status and Percentage of Normal Cells within Tumors | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2018-07-24T12:53:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22916147 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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Garcia-LinaresC.pdf | 760.91 kB | Adobe PDF | View/Open |
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