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http://hdl.handle.net/2445/126536
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DC Field | Value | Language |
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dc.contributor.author | Toll Abelló, Agustí | - |
dc.contributor.author | Salgado, Rocío | - |
dc.contributor.author | Espinet Solà, Blanca | - |
dc.contributor.author | Diaz-Lagares, Angel | - |
dc.contributor.author | Hernández Ruiz, Eugenia | - |
dc.contributor.author | Andrades, Evelyn | - |
dc.contributor.author | Sandoval, Juan | - |
dc.contributor.author | Esteller, Manel | - |
dc.contributor.author | Pujol, Ramon M. | - |
dc.contributor.author | Hernández Muñoz, Inmaculada | - |
dc.date.accessioned | 2018-11-28T11:02:11Z | - |
dc.date.available | 2018-11-28T11:02:11Z | - |
dc.date.issued | 2016-07-25 | - |
dc.identifier.issn | 1476-4598 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126536 | - |
dc.description.abstract | Background: Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer and frequently progresses from an actinic keratosis (AK), a sun-induced keratinocyte intraepithelial neoplasia (KIN). Epigenetic mechanisms involved in the phenomenon of progression from AK to cSCC remain to be elicited. Methods: Expression of microRNAs in sun-exposed skin, AK and cSCC was analysed by Agilent microarrays. DNA methylation of miR-204 promoter was determined by bisulphite treatment and pyrosequencing. Identification of miR-204 targets and pathways was accomplished in HaCat cells. Immunofluorescence and immunohistochemistry were used to analyze STAT3 activation and PTPN11 expression in human biopsies. Results: cSCCs display a marked downregulation of miR-204 expression when compared to AK. DNA methylation of miR-204 promoter was identified as one of the repressive mechanisms that accounts for miR-204 silencing in cSCC. In HaCaT cells miR-204 inhibits STAT3 and favours the MAPK signaling pathway, likely acting through PTPN11, a nuclear tyrosine phosphatase that is a direct miR-204 target. In non-peritumoral AK lesions, activated STAT3, as detected by pY705-STAT3 immunofluorescence, is retained in the membrane and cytoplasm compartments, whereas AK lesions adjacent to cSCCs display activated STAT3 in the nuclei. Conclusions: Our data suggest that miR-204 may act as a 'rheostat' that controls the signalling towards the MAPK pathway or the STAT3 pathway in the progression from AK to cSCC. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/s12943-016-0537-z | - |
dc.relation.ispartof | Molecular Cancer, 2016, vol. 15, num. 1, p. 53 | - |
dc.relation.uri | https://doi.org/10.1186/s12943-016-0537-z | - |
dc.rights | cc-by (c) Toll, Agustí et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Epigenètica | - |
dc.subject.classification | Càncer de pell | - |
dc.subject.classification | Ceratosi | - |
dc.subject.other | Epigenetics | - |
dc.subject.other | Skin cancer | - |
dc.subject.other | Keratosis | - |
dc.title | MiR-204 silencing in intraepithelial to invasive cutaneous squamous cell carcinoma progression | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 668660 | - |
dc.date.updated | 2018-11-28T11:02:11Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27457246 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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668660.pdf | 2.08 MB | Adobe PDF | View/Open |
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