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DC Field | Value | Language |
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dc.contributor.author | Rangel Salazar, Rubén | - |
dc.contributor.author | Wickström-Lindholm, Marie | - |
dc.contributor.author | Aguilar Salinas, Carlos A. | - |
dc.contributor.author | Alvarado-Caudillo, Yolanda | - |
dc.contributor.author | Døssing, Kristina B. V. | - |
dc.contributor.author | Esteller, Manel | - |
dc.contributor.author | Labourier, Emmanuel | - |
dc.contributor.author | Lund, Gertrud | - |
dc.contributor.author | Nielsen, Finn C. | - |
dc.contributor.author | Rodríguez-Ríos, Dalia | - |
dc.contributor.author | Solís Martínez, Martha O. | - |
dc.contributor.author | Wrobel, Katarzyna | - |
dc.contributor.author | Wrobel, Kazimierz | - |
dc.contributor.author | Zaina, Silvio | - |
dc.date.accessioned | 2018-11-30T09:41:42Z | - |
dc.date.available | 2018-11-30T09:41:42Z | - |
dc.date.issued | 2011-11-25 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126608 | - |
dc.description.abstract | Background: We previously showed that a VLDL-and LDL-rich mix of human native lipoproteins induces a set of repressive epigenetic marks, i. e. de novo DNA methylation, histone 4 hypoacetylation and histone 4 lysine 20 (H4K20) hypermethylation in THP-1 macrophages. Here, we: 1) ask what gene expression changes accompany these epigenetic responses; 2) test the involvement of candidate factors mediating the latter. We exploited genome expression arrays to identify target genes for lipoprotein-induced silencing, in addition to RNAi and expression studies to test the involvement of candidate mediating factors. The study was conducted in human THP-1 macrophages. Results: Native lipoprotein-induced de novo DNA methylation was associated with a general repression of various critical genes for macrophage function, including pro-inflammatory genes. Lipoproteins showed differential effects on epigenetic marks, as de novo DNA methylation was induced by VLDL and to a lesser extent by LDL, but not by HDL, and VLDL induced H4K20 hypermethylation, while HDL caused H4 deacetylation. The analysis of candidate factors mediating VLDL-induced DNA hypermethylation revealed that this response was: 1) surprisingly, mediated exclusively by the canonical maintenance DNA methyltransferase DNMT1, and 2) independent of the Dicer/microRNA pathway. Conclusions: Our work provides novel insights into epigenetic gene regulation by native lipoproteins. Furthermore, we provide an example of DNMT1 acting as a de novo DNA methyltransferase independently of canonical de novo enzymes, and show proof of principle that de novo DNA methylation can occur independently of a functional Dicer/micro-RNA pathway in mammals. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/1471-2164-12-582 | - |
dc.relation.ispartof | BMC Genomics, 2011, vol. 12, num. 582 | - |
dc.relation.uri | https://doi.org/10.1186/1471-2164-12-582 | - |
dc.rights | cc by (c) Rangel Salazar et al., 2011 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Regulació genètica | - |
dc.subject.classification | Lipoproteïnes | - |
dc.subject.other | Genetic regulation | - |
dc.subject.other | Lipoproteins | - |
dc.title | Human native lipoprotein-induced de novo DNA methylation is associated with repression of inflammatory genes in THP-1 macrophages | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 700259 | - |
dc.date.updated | 2018-07-24T12:58:11Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22118513 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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Rangel-SalazarR.pdf | 726.15 kB | Adobe PDF | View/Open |
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