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http://hdl.handle.net/2445/126873
Title: | Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping Of Exosome-like Vesicles |
Author: | Altadill, Tatiana Campoy, Irene Lanau, Lucia Gill, Kirandeep Rigau, Marina Gil Moreno, Antonio Reventós Puigjaner, Jaume Byers, Stephen W. Colás, Eva Cheema, Amrita K. |
Keywords: | Expressió gènica Marcadors bioquímics Gene expression Biochemical markers |
Issue Date: | 14-Mar-2016 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Identification of sensitive and specific biomarkers with clinical and translational utility will require smart experimental strategies that would augment expanding the breadth and depth of molecular measurements within the constraints of currently available technologies. Exosomes represent an information rich matrix to discern novel disease mechanisms that are thought to contribute to pathologies such as dementia and cancer. Although proteomics and transcriptomic studies have been reported using Exosomes-Like Vesicles (ELVs) from different sources, exosomal metabolome characterization and its modulation in health and disease remains to be elucidated. Here we describe methodologies for UPLC-ESI-MS based small molecule profiling of ELVs from human plasma and cell culture media. In this study, we present evidence that indeed ELVs carry a rich metabolome that could not only augment the discovery of low abundance biomarkers but may also help explain the molecular basis of disease progression. This approach could be easily translated to other studies seeking to develop predictive biomarkers that can subsequently be used with simplified targeted approaches. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0151339 |
It is part of: | PLoS One, 2016, vol. 11, num. 3, p. e0151339 |
URI: | http://hdl.handle.net/2445/126873 |
Related resource: | https://doi.org/10.1371/journal.pone.0151339 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Publicacions de projectes de recerca finançats per la UE |
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