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Title: | Macrophage-derived Lipocalin-2 contributes to ischemic resistance mechanisms by protecting from renal injury |
Author: | Jung, Michaela Brüne, Bernhard, 1957- Hotter Corripio, Georgina Sola Martínez, Anna |
Keywords: | Insuficiència renal Macròfags Renal insufficiency Macrophages |
Issue Date: | 25-Feb-2016 |
Publisher: | Nature Publishing Group |
Abstract: | Renal ischemia-reperfusion injury triggers an inflammatory response associated to infiltrating macrophages which determines the further outcome of disease. Brown Norway rats are known to show endogenous resistance to ischemia-induced renal damage. By contrast, Sprague Dawley rats exhibit a higher susceptibility to ischemic injury. In order to ascertain cytoprotective mechanisms, we focused on the implication of lipocalin-2 protein in main resistance mechanisms in renal ischemia/reperfusion injury by using adoptive macrophage administration, genetically modified ex vivo either to overexpress or to knockdown lipocalin-2. In vitro experiments with bone marrow-derived macrophages both from Brown Norway rats and from Sprague Dawley rats under hypoxic conditions showed endogenous differences regarding cytokine and lipocalin-2 expression profile in the two strains. Most interestingly, we observed that macrophages of the resistant strain express significantly more lipocalin-2. In vivo studies showed that tubular epithelial cell apoptosis and renal injury significantly increased and reparative markers decreased in Brown Norway rats after injection of lipocalin-2-knockdown macrophages, while the administration of lipocalin-2-overexpressing cells significantly decreased Sprague Dawley susceptibility. These data point to a crucial role of macrophage-derived lipocalin-2 in endogenous cytoprotective mechanisms. We conclude that expression of lipocalin-2 in tissue-infiltrating macrophages is pivotal for kidney-intrinsic cytoprotective pathways during ischemia reperfusion injury. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/srep21950 |
It is part of: | Scientific Reports, 2016, vol. 6 |
URI: | http://hdl.handle.net/2445/126879 |
Related resource: | https://doi.org/10.1038/srep21950 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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