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Title: | Inflammatory dysregulation of monocytes in pediatric patients with obsessive-compulsive disorder |
Author: | Rodríguez Ferret, Natalia Morer Liñán, Astrid González-Navarro, Europa Azucena Serra Pagès, Carles Boloc, Daniel Torres, Teresa García Cerro, Susana Mas Herrero, Sergi Gassó Astorga, Patricia Lázaro García, Luisa |
Keywords: | Psiquiatria infantil Inflamació Neurosi obsessiva Child psychiatry Inflammation Obsessive-compulsive disorder |
Issue Date: | 28-Dec-2017 |
Publisher: | BioMed Central |
Abstract: | BACKGROUND: Although the exact etiology of obsessive-compulsive disorder (OCD) is unknown, there is growing evidence of a role for immune dysregulation in the pathophysiology of the disease, especially in the innate immune system including the microglia. To test this hypothesis, we studied inflammatory markers in monocytes from pediatric patients with OCD and from healthy controls. METHODS: We determined the percentages of total monocytes, CD16+ monocytes, and classical (CD14highCD16-), intermediate (CD14highCD16low), and non-classical (CD14lowCD16high) monocyte subsets in 102 patients with early-onset OCD and in 47 healthy controls. Moreover, proinflammatory cytokine production (GM-CSF, IL-1β, IL-6, IL-8, and TNF-α) was measured by multiplex Luminex analysis in isolated monocyte cultures, in basal conditions, after exposure to lipopolysaccharide (LPS) to stimulate immune response or after exposure to LPS and the immunosuppressant dexamethasone. RESULTS: OCD patients had significantly higher percentages of total monocytes and CD16+ monocytes than healthy controls, mainly due to an increase in the intermediate subset but also in the non-classical monocytes. Monocytes from OCD patients released higher amounts of GM-CSF, IL-1β, IL-6, IL-8, and TNF-α than healthy controls after exposure to LPS. However, there were no significant differences in basal cytokine production or the sensitivity of monocytes to dexamethasone treatment between both groups. Based on monocyte subset distribution and cytokine production after LPS stimulation, patients receiving psychoactive medications seem to have an intermediate inflammatory profile, that is, lower than non-medicated OCD individuals and higher than healthy controls. CONCLUSIONS: These results strongly support the involvement of an enhanced proinflammatory innate immune response in the etiopathogenesis of early-onset OCD. |
Note: | Reproducció del document publicat a: https://doi.org/10.1186/s12974-017-1042-z |
It is part of: | Journal of Neuroinflammation, 2017, vol. 14, num. 1, p. 261 |
URI: | http://hdl.handle.net/2445/127107 |
Related resource: | https://doi.org/10.1186/s12974-017-1042-z |
ISSN: | 1742-2094 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (Fonaments Clínics) |
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