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http://hdl.handle.net/2445/127778
Title: | Bacterial lysis through interference with peptidoglycan synthesis increases biofilm formation by nontypeable haemophilus influenzae |
Author: | Martí Martí, Sara Puig Pitarch, Carmen Merlos, Alexandra Viñas, Miquel Jonge, Marien I. de Liñares Louzao, Josefina Ardanuy Tisaire, María Carmen Langereis, Jeroen D. |
Keywords: | Otitis mitjana Malalties infeccioses en els infants Pneumònia Antibiòtics Síntesi de pèptids Biofilms ADN Otitis media Communicable diseases in children Pneumonia Antibiotics Peptide synthesis Biofilms DNA |
Issue Date: | 18-Jan-2017 |
Publisher: | American Society for Microbiology |
Abstract: | Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen that mainly causes otitis media in children and community-acquired pneumonia or exacerbations of chronic obstructive pulmonary disease in adults. A large variety of studies suggest that biofilm formation by NTHi may be an important step in the pathogenesis of this bacterium. However, the underlying mechanisms involved in this process are poorly elucidated. In this study, we used a transposon mutant library to identify bacterial genes involved in biofilm formation. The growth and biofilm formation of 4,172 transposon mutants were determined, and the involvement of the identified genes in biofilm formation was validated in in vitro experiments. Here, we present experimental data showing that increased bacterial lysis, through interference with peptidoglycan synthesis, results in elevated levels of extracellular DNA, which increased biofilm formation. Interestingly, similar results were obtained with subinhibitory concentrations of β-lactam antibiotics, known to interfere with peptidoglycan synthesis, but such an effect does not appear with other classes of antibiotics. These results indicate that treatment with β-lactam antibiotics, especially for β-lactam-resistant NTHi isolates, might increase resistance to antibiotics by increasing biofilm formation. |
Note: | Reproducció del document publicat a: https://doi.org/10.1128/mSphere.00329-16 |
It is part of: | mSphere, 2017, vol. 2, num. 1, p. e00329-16 |
URI: | http://hdl.handle.net/2445/127778 |
Related resource: | https://doi.org/10.1128/mSphere.00329-16 |
ISSN: | 2379-5042 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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