Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/130339
Title: | Effects of two different decellularization routes on the mechanical properties of decellularized lungs |
Author: | Urbano, Jessica Julioti Palma, Renata Kelly da Lima Osório, Flávia de Fratini, Paula Lopes Guimaraes, Leticia Uriarte, Juan José Heineck Alvarenga, Leticia Miglino, Maria Angelica Paula Vieira, Rodolfo de Araujo Prates, Renato Navajas Navarro, Daniel Farré Ventura, Ramon Franco Oliveira, Luis Vicente |
Keywords: | Pulmó Bioenginyeria Models animals en la investigació Lung Bioengineering Animal models in research |
Issue Date: | 1-Jun-2017 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Considering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare the acellular lung mechanical properties when using two different routes through the trachea and pulmonary artery for the decellularization process. This study was performed by using the lungs excised from 30 healthy male C57BL/6 mice, which were divided into 3 groups: tracheal decellularization (TDG), perfusion decellularization (PDG), and control groups (CG). Both decellularized groups were subjected to decellularization protocol with a solution of 1% sodium dodecyl sulfate. The behaviour of mechanical properties of the acellular lungs was measured after decellularization process. Static (Est) and dynamic (Edyn) elastances were obtained by the end-inspiratory occlusion method. TDG and PDG showed reduced Est and Edyn elastances after lung decellularization. Scanning electron microscopy showed no structural changes after lung decellularization of the TDG and PDG. In conclusion, was demonstrated that there is no significant difference in the behaviour of mechanical properties and extracellular matrix of the decellularized lungs by using two different routes through the trachea and pulmonary artery. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0178696 |
It is part of: | PLoS One, 2017, vol. 12, num. 6, p. e0178696 |
URI: | https://hdl.handle.net/2445/130339 |
Related resource: | https://doi.org/10.1371/journal.pone.0178696 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
676590.pdf | 973.82 kB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License