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DC Field | Value | Language |
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dc.contributor.author | Gandolfini, Ilaria | - |
dc.contributor.author | Crespo, Elena | - |
dc.contributor.author | Baweja, Mukta | - |
dc.contributor.author | Jarque, Marta | - |
dc.contributor.author | Donadei, Chiara | - |
dc.contributor.author | Luque, Sergio | - |
dc.contributor.author | Montero Pérez, Núria | - |
dc.contributor.author | Allesina, Anna | - |
dc.contributor.author | Perin, Laura | - |
dc.contributor.author | Maggiore, Umberto | - |
dc.contributor.author | Cravedi, Paolo | - |
dc.contributor.author | Bestard Matamoros, Oriol | - |
dc.date.accessioned | 2019-03-19T10:59:46Z | - |
dc.date.available | 2019-03-19T10:59:46Z | - |
dc.date.issued | 2018-07-30 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/130524 | - |
dc.description.abstract | Donor-specific (d-sp) interferon gamma enzyme-linked immunosorbent spot (d-sp ELISPOT)and Panel of reactive T-cell (PRT) ELISPOT assays have been developed to detect alloreactive memory T (Tmem) cells in order to estimate the risk of acute rejection after kidney transplantation. Adding IL15 to the PRT assay (PRT+IL15) may uncover the presence of pathogenic alloreactive CD28-Tmem. Face-to-face comparisons of these assays have not been done yet. We performed pre-transplant d-sp ELISPOT and PRT assays (±IL15, against six B-cell lines) in 168 consecutive kidney transplant recipients and evaluated the multivariable-adjusted associations with biopsy-proven acute rejection (BPAR), de novo donor-specific antibodies (DSA), and eGFR decline over a 48-month follow-up period. D-sp ELISPOT was positive in 81 (48%) subjects, while 71 (42%) and 81 (48%) subjects displayed positive PRT and PRT+IL15, respectively.Their median [interquartile range] numerical test result was 23 [6±65], 18 [8±37], and 26 [10±45] spots/3x105 PBMCs, respectively. The number of PRT spots were weakly correlated with those of d-sp ELISPOT, but highly correlated with PRT+IL15 (rho = 0.96, P<0.001). d-sp ELISPOT, but not PRT (±IL15) was independently associated with BPAR (adjusted Odds Ratio of BPAR associated with d-sp ELISPOT positivity: 4.20 [95%CI: 1.06 to 21.73; P = 0.041]). Unlike d-sp ELISPOT, median PRT and PRT+IL15 were independently associated with higher Δ3-48month eGFR decline post-transplantation (for both assays, about -3mL/min/1.73m2 per one standard deviation unit increase in the spot number). Pre-transplant T-cell immune-monitoring using d-sp ELISPOT and PRT assays identifies kidney transplant candidates at high risk of BPAR and worse kidney allograft progression. | - |
dc.format.extent | 26 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0200696 | - |
dc.relation.ispartof | PLoS One, 2018, vol. 13, num. 7, p. e200696 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0200696 | - |
dc.rights | cc-by (c) Gandolfini, Ilaria et al., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Trasplantament renal | - |
dc.subject.classification | Immunologia de la trasplantació | - |
dc.subject.classification | Malalties del ronyó | - |
dc.subject.other | Kidney transplantation | - |
dc.subject.other | Transplantation immunology | - |
dc.subject.other | Kidney diseases | - |
dc.title | Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 685166 | - |
dc.date.updated | 2019-03-19T10:59:47Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30059561 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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