Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/130563
Title: | Dynamic photopolymerization produces complex microstructures on hydrogels in a moldless approach to generate a 3D intestinal tissue model |
Author: | Garcia Castaño, Albert García Díaz, María Torras, Núria Altay, Gizem Comelles Pujadas, Jordi Martínez Fraiz, Elena |
Keywords: | Teixits (Histologia) Enginyeria de teixits Epiteli Gels (Farmàcia) Tissues Tissue engineering Epithelium Gels (Pharmacy) |
Issue Date: | 25-Feb-2019 |
Publisher: | Institute of Physics Pub. |
Abstract: | Epithelial tissues contain three-dimensional (3D) complex microtopographies that are essential for proper performance. These microstructures provide cells with the physicochemical cues needed to guide their self-organization into functional tissue structures. However, most in vitro models do not implement these 3D architectural features. The main problem is the availability of simple fabrication techniques that can reproduce the complex geometries found in native tissues on the soft polymeric materials required as cell culture substrates. In this study reaction-diffusion mediated photolithography is used to fabricate 3D microstructures with complex geometries on poly(ethylene glycol)-based hydrogels in a single step and moldless approach. By controlling fabrication parameters such as the oxygen diffusion/depletion timescales, the distance to the light source and the exposure dose, the dimensions and geometry of the microstructures can be well-defined. In addition, copolymerization of poly(ethylene glycol) with acrylic acid improves control of the dynamic reaction-diffusion processes that govern the free-radical polymerization of highly-diluted polymeric solutions. Moreover, acrylic acid allows adjusting the density of cell adhesive ligands while preserving the mechanical properties of the hydrogels. The method proposed is a simple, single-step, and cost-effective strategy for producing models of intestinal epithelium that can be easily integrated into standard cell culture platform |
Note: | Versió postprint del document publicat a: https://doi.org/10.1088/1758-5090/ab0478 |
It is part of: | Biofabrication, 2019, vol. 11, num. 2, p. 025007 |
URI: | http://hdl.handle.net/2445/130563 |
Related resource: | https://doi.org/10.1088/1758-5090/ab0478 |
ISSN: | 1758-5082 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
685875.pdf | 1.28 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.