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Title: Rapid biolayer interferometry measurements of urinary CXCL9 to detect cellular infiltrates noninvasively after kidney transplantation
Author: Gandolfini, Ilaria
Harris, Cynthia
Abecassis, Michael
Anderson, Lisa
Bestard Matamoros, Oriol
Comai, Giorgia
Cravedi, Paolo
Cremaschi, Elena
Duty, J. Andrew
Florman, Sander
Friedewald, John
La Manna, Gaetano
Maggiore, Umberto
Moran, Thomas
Piotti, Giovanni
Purroy, Carolina
Jarque, Marta
Nair, Vinay
Shapiro, Ron
Reid-Adam, Jessica
Heeger, Peter S.
Keywords: Rebuig (Biologia)
Trasplantament renal
Graft rejection
Kidney transplantation
Issue Date: Nov-2017
Publisher: Elsevier
Abstract: Introduction: measuring the chemokine CXCL9 in urine by enzyme-linked immunosorbent assay (ELISA) can diagnose acute cellular rejection (ACR) noninvasively after kidney transplantation, but the required 12- to 24-hour turnaround time is not ideal for rapid, clinical decision-making. Methods: we developed a biolayer interferometry (BLI)−based assay to rapidly measure urinary CXCL9 in <1 hour. We validated this new assay versus standard ELISA in 86 urine samples from kidney transplantation recipients with various diagnoses. We then used BLI to analyze samples from 56 kidney transplantation recipients, including 46 subjects who experienced an acute rise in serum creatinine associated with biopsy-proven ACR (n = 22), subclinical rejection (n = 15), or no infiltrates (n = 9), and 10 stable kidney transplantation recipients with surveillance biopsies. To assess its usefulness in detecting adequacy of therapy we serially measured serum creatinine and urinary CXCL9 in 6 subjects after treatment for ACR, and correlated the results with histological diagnoses on follow-up biopsies. Results: BLI accurately and reproducibly detected urinary CXCL9 in <1 hour. BLI-based results showed that urinary CXCL9 was >200 pg/ml in subjects with ACR and ≤100 pg/ml in subjects with stable kidney function without cellular infiltrates. In samples obtained after treatment for ACR, BLI CXCL9 measurements detected biopsy-proven intragraft infiltrates despite treatment-induced reduction in serum creatinine. Discussion: together, our proof-of-principle results demonstrate that BLI-based urinary CXCL9 detection has potential as a point-of-care noninvasive biomarker to diagnose and guide therapy for ACR in kidney transplantation recipients.
Note: Reproducció del document publicat a:
It is part of: Kidney International Reports, 2017, vol. 2, num. 6, p. 1186-1193
Related resource:
ISSN: 2468-0249
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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