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Title: Identification and characterization of the zebrafish ClC-2 chloride channel orthologs
Author: Pérez Rius, Carla
Gaitán-Peñas, Héctor
Estévez Povedano, Raúl
Barrallo-Gimeno, Alejandro
Keywords: Canals de clorur
Seqüència de nucleòtids
Metabolisme de proteïnes
Peix zebra
Chloride channels
Nucleotide sequence
Protein metabolism
Zebra danio
Issue Date: Aug-2015
Publisher: Springer Verlag
Abstract: ClC-2 is a Cl− channel that belongs to the CLC family of chloride channel/transport proteins. ClC-2 molecular role is not clear, and Clcn2 knockout mice develop blindness, sterility, and leukodystrophy by unknown reasons. ClC-2 is associated in the brain with the adhesion molecule GlialCAM, which is defective in a type of leukodystrophy, involving ClC-2 in the homeostasis of myelin. To get more insight into the functions of ClC-2, we have identified in this work the three ClC-2 orthologs in zebrafish. clcn2a and clcn2b resulted from the teleost-specific whole genome duplication, while clcn2c arose from a gene duplication from clcn2b. The expression patterns in adult tissues and embryos of zebrafish clcn2 paralogs support their subfunctionalization after the duplications, with clcn2a being enriched in excitable tissues and clcn2c in ionocytes. All three zebrafish clc-2 proteins interact with human GLIALCAM, that is able to target them to cell junctions, as it does with mammalian ClC-2. We could detect clc-2a and clc-2b inward rectified chloride currents with different voltage-dependence and kinetics in Xenopus oocytes, while clc-2c remained inactive. Interestingly, GlialCAM proteins did not modify clc-2b inward rectification. Then, our work extends the repertoire of ClC-2 proteins and provides new tools for structure-function and physiology studies.
Note: Versió postprint del document publicat a:
It is part of: Pflugers Archiv-European Journal of Physiology, 2015, vol. 467, num. 8, p. 1769-1781
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ISSN: 0031-6768
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Ciències Fisiològiques)

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