Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/134299
Title: | The atrial natriuretic peptide and guanylyl cyclase-A system modulates pancreatic beta-cell function |
Author: | Ropero, Ana B. Soriano, Sergi Tudurí, Eva Marroquí, Laura Téllez i Besolí, Noèlia Gassner, Birgit Juan-Picó, Pablo Montanya Mias, Eduard Quesada, Ivan Kuhn, Michaela Nadal, Angel |
Keywords: | Fisiologia Secreció Insulina Pèptids Physiology Secretion Insulin Peptides |
Issue Date: | Aug-2010 |
Publisher: | Association for the Study of Internal Secretions |
Abstract: | Atrial natriuretic peptide (ANP) and its guanylyl cyclase-A (GC-A) receptor are being involved in metabolism, although their role in the endocrine pancreas is still greatly unknown. The aim of this work is to study a possible role for the ANP/GC-A system in modulating pancreatic beta-cell function. The results presented here show a direct effect of the GC-A receptor in regulating glucose-stimulated insulin secretion (GSIS) and beta-cell mass. GC-A activation by its natural ligand, ANP, rapidly blocked ATP-dependent potassium (K(ATP)) channel activity, increased glucose-elicited Ca(2+) signals, and enhanced GSIS in islets of Langerhans. The effect in GSIS was inhibited in islets from GC-A knockout (KO) mice. Pancreatic islets from GC-A KO mice responded to increasing glucose concentrations with enhanced insulin secretion compared with wild type (WT). Remarkably, islets from GC-A KO mice were smaller, presented lower beta-cell mass and decreased insulin content. However, glucose-induced Ca(2+) response was more vigorous in GC-A KO islets, and basal K(ATP) channel activity in GC-A KO beta-cells was greatly diminished compared with WT. When protein levels of the two K(ATP) channel constitutive subunits sulfonylurea receptor 1 and Inward rectifier potassium channel 6.2 were measured, both were diminished in GC-A KO islets. These alterations on beta-cell function were not associated with disruption of glucose tolerance or insulin sensitivity in vivo. Glucose and insulin tolerance tests were similar in WT and GC-A KO mice. Our data suggest that the ANP/GC-A system may have a modulating effect on beta-cell function. |
Note: | Reproducció del document publicat a: https://doi.org/10.1210/en.2010-0119 |
It is part of: | Endocrinology, 2010, vol. 151, num. 8, p. 3665-3674 |
URI: | http://hdl.handle.net/2445/134299 |
Related resource: | https://doi.org/10.1210/en.2010-0119 |
ISSN: | 0013-7227 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
630888.pdf | 408.05 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.