Please use this identifier to cite or link to this item:
Title: 3D Electrophoresis-assisted lithography (3DEAL): 3D molecular printing to create functional patterns and anisotropic hydrogels
Author: Aguilar, Juan P.
Lipka, Michal
Primo, Gastón A.
Licon Bernal, Edxon Eduardo
Fernández Pradas, Juan Marcos
Yaroshchuk, Andriy
Albericio Palomera, Fernando
Mata, Álvaro
Keywords: Electroforesi
Impressió 3D
Three-dimensional printing
Issue Date: 11-Apr-2018
Publisher: Wiley-VCH
Abstract: The ability to easily generate anisotropic hydrogel environments made from functional molecules with microscale resolution is an exciting possibility for the biomaterials community. This study reports a novel 3D electrophoresis‐assisted lithography (3DEAL) platform that combines elements from proteomics, biotechnology, and microfabrication to print well‐defined 3D molecular patterns within hydrogels. The potential of the 3DEAL platform is assessed by patterning immunoglobulin G, fibronectin, and elastin within nine widely used hydrogels and characterizing pattern depth, resolution, and aspect ratio. Furthermore, the technique's versatility is demonstrated by fabricating complex patterns including parallel and perpendicular columns, curved lines, gradients of molecular composition, and patterns of multiple proteins ranging from tens of micrometers to centimeters in size and depth. The functionality of the printed molecules is assessed by culturing NIH‐3T3 cells on a fibronectin‐patterned polyacrylamide‐collagen hydrogel and selectively supporting cell growth. 3DEAL is a simple, accessible, and versatile hydrogel‐patterning platform based on controlled molecular printing that may enable the development of tunable, chemically anisotropic, and hierarchical 3D environments.
Note: Versió postprint del document publicat a:
It is part of: Advanced Functional Materials, 2018, vol. 28, num. 15, p. 1703014
Related resource:
ISSN: 1616-301X
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)
Articles publicats en revistes (Física Aplicada)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Files in This Item:
File Description SizeFormat 
676567.pdf12.7 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.