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Title: Proteomics study of human cord blood reticulocyte-derived exosomes
Author: Díaz Varela, Míriam
Menezes Neto, Armando de
Pérez Zsolt, Daniel
Gámez Valero, Ana
Seguí Barber, Joan
Izquierdo Useros, Nuria
Martínez Picado, Francisco Javier
Fernández-Becerra, Carmen
Portillo Obando, Hernando A. del
Keywords: Cèl·lules dendrítiques
Dendritic cells
Issue Date: 19-Sep-2018
Publisher: Nature Publishing Group
Abstract: Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.
Note: Reproducció del document publicat a: 10.1038/s41598-018-32386-2
It is part of: Scientific Reports, 2018, vol. 8, p. 14046
Related resource: 10.1038/s41598-018-32386-2
ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (ISGlobal)

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