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http://hdl.handle.net/2445/134938
Title: | Characterization of glycoproteins by capillary electrophoresis electrospray mass spectrometry (CE-ESI-MS). Applications to diagnosis in biomedicine |
Author: | Barbosa Torralbo, José Sanz Nebot, María Victoria Benavente Moreno, Fernando J. (Julián) Giménez López, Estela |
Keywords: | Electroforesi capil·lar Glicoproteïnes Espectrometria de masses Proteïnes Capillary electrophoresis Glycoproteins Mass spectrometry Proteins |
Issue Date: | 1-Jun-2006 |
Abstract: | Glycosylation is the most common posttranslational modification in proteins and the carbohydrates participate in many biological processes. The number and type of glycoforms for a certain glycoprotein may change as a consequence of pathological processes. In our work a method for the separation of transferrin sialoforms has been developed, that permits the diagnostic of Congenital Disorders of Glycosylation (CDG) using a polybrene-dextran sulphate coating and CE-ESI-TOF methodologies. In order to improve the sensitivity the use of solid-phase extraction coupled on-line to CE-ESI-MS is studied and the SPE-CE-ESI-MS developed methods are applied for the characterization of rHuEPO glycoforms. The achieved separation and the high mass-resolving power of flight (TOF) mass detection allows to establish the most probable rHuEPO glycoforms. |
Note: | http://dergi.fabad.org.tr/pdf/volum31/issue2/63-69.pdf |
It is part of: | FABAD Journal of Pharmaceutical Sciences, 2006, vol. 31, p. 63-69 |
URI: | http://hdl.handle.net/2445/134938 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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551918.pdf | 1.82 MB | Adobe PDF | View/Open |
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