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DC Field | Value | Language |
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dc.contributor.author | Martin, Franz | - |
dc.contributor.author | Andreu, Etelvina | - |
dc.contributor.author | Rovira, Juan Manuel | - |
dc.contributor.author | Pertusa, Jose A. G. | - |
dc.contributor.author | Raurell, Mercè | - |
dc.contributor.author | Ripoll, Cristina | - |
dc.contributor.author | Sanchez-Andres, Juan Vicente | - |
dc.contributor.author | Montanya Mias, Eduard | - |
dc.contributor.author | Soria, Bernat | - |
dc.date.accessioned | 2019-06-13T14:40:12Z | - |
dc.date.available | 2019-06-13T14:40:12Z | - |
dc.date.issued | 1999-10 | - |
dc.identifier.issn | 0012-1797 | - |
dc.identifier.uri | http://hdl.handle.net/2445/135008 | - |
dc.description.abstract | Increased beta-cell sensitivity to glucose precedes the loss of glucose-induced insulin secretion in diabetic animals. Changes at the level of beta-cell glucose sensor have been described in these situations, but it is not clear whether they fully account for the increased insulin secretion. Using a euglycemic-normolipidemic 60% pancreatectomized (60%-Px) mouse model, we have studied the ionic mechanisms responsible for increased beta-cell glucose sensitivity. Two weeks after Px (Px14 group), Px mice maintained normoglycemia with a reduced beta-cell mass (0.88 +/- 0.18 mg) compared with control mice (1.41 +/- 0.21 mg). At this stage, the dose-response curve for glucose-induced insulin release showed a significant displacement to the left (P < 0.001). Islets from the Px14 group showed oscillatory electrical activity and cytosolic Ca2+ ([Ca2+]i) oscillations in response to glucose concentrations of 5.6 mmol/l compared with islets from the control group at 11.1 mmol/l. All the above changes were fully reversible both in vitro (after 48-h culture of islets from the Px14 group) and in vivo (after regeneration of beta-cell mass in islets studied 60 days after Px). No significant differences in the input resistance and ATP inhibition of ATP-sensitive K+ (K(ATP)) channels were found between beta-cells from the Px14 and control groups. The dose-response curve for glucose-induced MTT (C,N-diphenyl-N''-4,5-dimethyl thiazol 2 yl tetrazolium bromide) reduction showed a significant displacement to the left in islets from the Px14 group (P < 0.001). These results indicate that increased glucose sensitivity in terms of insulin secretion and Ca2+ signaling was not due to intrinsic modifications of K(ATP) channel properties, and suggest that the changes are most likely to be found in the glucose metabolism. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Diabetes Association | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.2337/diabetes.48.10.1954 | - |
dc.relation.ispartof | Diabetes, 1999, vol. 48, num. 10, p. 1954-1961 | - |
dc.relation.uri | https://doi.org/10.2337/diabetes.48.10.1954 | - |
dc.rights | cc-by-nc-nd (c) American Diabetes Association, 1999 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.subject.classification | Glucosa | - |
dc.subject.classification | Fisiologia | - |
dc.subject.classification | Illots de Langerhans | - |
dc.subject.classification | Ratolins (Animals de laboratori) | - |
dc.subject.classification | Cèl·lules B | - |
dc.subject.other | Glucose | - |
dc.subject.other | Physiology | - |
dc.subject.other | Islands of Langerhans | - |
dc.subject.other | Mice (Laboratory animals) | - |
dc.subject.other | B cells | - |
dc.title | Mechanisms of glucosa hypersensitivity in ß-cells from normoglycemic, partially pancreatectomized mice | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 537255 | - |
dc.date.updated | 2019-06-13T14:40:12Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 10512359 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) |
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