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Title: Obstructive apneas induce early activation of mesenchymal stem cells and enhancement of endothelial wound healing
Author: Carreras, Alba
Rojas, Mauricio
Tsapikouni, Theodora
Montserrat Canal, José Ma.
Navajas Navarro, Daniel
Farré Ventura, Ramon
Keywords: Trastorns respiratoris
Fisiologia animal
Respiration disorders
Physiology, Comparative
Issue Date: 6-Jul-2010
Publisher: BioMed Central
Abstract: Background: The aim was to test the hypothesis that the blood serum of rats subjected to recurrent airway obstructions mimicking obstructive sleep apnea (OSA) induces early activation of bone marrow-derived mesenchymal stem cells (MSC) and enhancement of endothelial wound healing. Methods: We studied 30 control rats and 30 rats subjected to recurrent obstructive apneas (60 per hour, lasting 15 s each, for 5 h). The migration induced in MSC by apneic serum was measured by transwell assays. MSC-endothelial adhesion induced by apneic serum was assessed by incubating fluorescent-labelled MSC on monolayers of cultured endothelial cells from rat aorta. A wound healing assay was used to investigate the effect of apneic serum on endothelial repair. Results: Apneic serum showed significant increase in chemotaxis in MSC when compared with control serum: the normalized chemotaxis indices were 2.20 +- 0.58 (m +- SE) and 1.00 +- 0.26, respectively (p < 0.05). MSC adhesion to endothelial cells was greater (1.75 +- 0.14 -fold; p < 0.01) in apneic serum than in control serum. When compared with control serum, apneic serum significantly increased endothelial wound healing (2.01 +- 0.24 -fold; p < 0.05). Conclusions: The early increases induced by recurrent obstructive apneas in MSC migration, adhesion and endothelial repair suggest that these mechanisms play a role in the physiological response to the challenges associated to OSA.
Note: Reproducció del document publicat a
It is part of: Respiratory Research 2010, 11:91, p. 1-7
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ISSN: 1465-9921
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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