Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/138522
Title: A three-protein charge zipper stabilizes a protein complex modulating bacterial gene silencing
Author: Cordeiro, Tiago N.
García, Jesús
Bernadó Peretó, Pau
Millet Aguilar-Galindo, Òscar
Pons Vallès, Miquel
Keywords: Electroestàtica
Ressonància magnètica nuclear
Proteïnes
Electrostatics
Nuclear magnetic resonance
Proteins
Issue Date: 28-Aug-2015
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: The Hha/YmoA nucleoid-associated proteins help selectively silence horizontally acquired genetic material, including pathogenicity and antibiotic resistance genes and their maintenance in the absence of selective pressure. Members of the Hha family contribute to gene silencing by binding to the N-terminal dimerization domain of H-NS and modifying its selectivity. Hha-like proteins and the H-NS N-terminal domain are unusually rich in charged residues, and their interaction is mostly electrostatic-driven but, nonetheless, highly selective. The NMRbased structural model of the complex between Hha/YmoA and the H-NS N-terminal dimerization domain reveals that the origin of the selectivity is the formation of a three-protein charge zipper with interdigitated complementary charged residues from Hha and the two units of the H-NS dimer. The free form of YmoA shows collective microsecond-millisecond dynamics that can by measured by NMR relaxation dispersion experiments and shows a linear dependence with the salt concentration. The number of residues sensing the collective dynamics and the population of the minor form increased in the presence of H-NS. Additionally, a single residue mutation in YmoA (D43N) abolished H-NS binding and the dynamics of the apo-form, suggesting the dynamics and binding are functionally related
Note: Reproducció del document publicat a: https://doi.org/10.1074/jbc.M114.630400
It is part of: Journal of Biological Chemistry, 2015, vol. 290, p. 21200-21212
URI: http://hdl.handle.net/2445/138522
Related resource: https://doi.org/10.1074/jbc.M114.630400
ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)
Publicacions de projectes de recerca finançats per la UE

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