Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/138522
Title: | A three-protein charge zipper stabilizes a protein complex modulating bacterial gene silencing |
Author: | Cordeiro, Tiago N. García, Jesús Bernadó Peretó, Pau Millet Aguilar-Galindo, Òscar Pons Vallès, Miquel |
Keywords: | Electroestàtica Ressonància magnètica nuclear Proteïnes Electrostatics Nuclear magnetic resonance Proteins |
Issue Date: | 28-Aug-2015 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | The Hha/YmoA nucleoid-associated proteins help selectively silence horizontally acquired genetic material, including pathogenicity and antibiotic resistance genes and their maintenance in the absence of selective pressure. Members of the Hha family contribute to gene silencing by binding to the N-terminal dimerization domain of H-NS and modifying its selectivity. Hha-like proteins and the H-NS N-terminal domain are unusually rich in charged residues, and their interaction is mostly electrostatic-driven but, nonetheless, highly selective. The NMRbased structural model of the complex between Hha/YmoA and the H-NS N-terminal dimerization domain reveals that the origin of the selectivity is the formation of a three-protein charge zipper with interdigitated complementary charged residues from Hha and the two units of the H-NS dimer. The free form of YmoA shows collective microsecond-millisecond dynamics that can by measured by NMR relaxation dispersion experiments and shows a linear dependence with the salt concentration. The number of residues sensing the collective dynamics and the population of the minor form increased in the presence of H-NS. Additionally, a single residue mutation in YmoA (D43N) abolished H-NS binding and the dynamics of the apo-form, suggesting the dynamics and binding are functionally related |
Note: | Reproducció del document publicat a: https://doi.org/10.1074/jbc.M114.630400 |
It is part of: | Journal of Biological Chemistry, 2015, vol. 290, p. 21200-21212 |
URI: | http://hdl.handle.net/2445/138522 |
Related resource: | https://doi.org/10.1074/jbc.M114.630400 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) Publicacions de projectes de recerca finançats per la UE |
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655712.pdf | 4.05 MB | Adobe PDF | View/Open |
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