Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/139271
Title: | TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4 |
Author: | Kuipers, Arthur J. Middelbeek, Jeroen Vrenken, Kirsten Pérez González, Carlos Poelmans, Geert Klarenbeek, Jeffrey Jalink, Kees Trepat Guixer, Xavier Leeuwen, Frank N. van |
Keywords: | Càncer de mama Citosquelet Tumors Breast cancer Cytoskeleton Tumors |
Issue Date: | Jul-2018 |
Publisher: | Elsevier B.V. |
Abstract: | Mechanically induced signaling pathways are important drivers of tumor progression. However, if and how mechanical signals affect metastasis or therapy response remains poorly understood. We previously found that the channel-kinase TRPM7, a regulator of cellular tension implicated in mechano-sensory processes, is required for breast cancer metastasis in vitro and in vivo. Here, we show that TRPM7 contributes to maintaining a mesenchymal phenotype in breast cancer cells by tensional regulation of the EMT transcription factor SOX4. The functional consequences of SOX4 knockdown closely mirror those produced by TRPM7 knockdown. By traction force measurements, we demonstrate that TRPM7 reduces cytoskeletal tension through inhibition of myosin II activity. Moreover, we show that SOX4 expression and downstream mesenchymal markers are inversely regulated by cytoskeletal tension and matrix rigidity. Overall, our results identify SOX4 as a transcription factor that is uniquely sensitive to cellular tension and indicate that TRPM7 may contribute to breast cancer progression by tensional regulation of SOX4. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.bbadis.2018.04.017 |
It is part of: | Biochimica et Biophysica Acta, 2018, vol. 1864, num. 7, p. 2409-2419 |
URI: | https://hdl.handle.net/2445/139271 |
Related resource: | https://doi.org/10.1016/j.bbadis.2018.04.017 |
ISSN: | 0006-3002 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Publicacions de projectes de recerca finançats per la UE |
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