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DC Field | Value | Language |
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dc.contributor.author | López Gutiérrez, Borja | - |
dc.contributor.author | Cova, Marta | - |
dc.contributor.author | Izquierdo Lázaro, Luis | - |
dc.date.accessioned | 2019-10-29T11:23:53Z | - |
dc.date.available | 2019-10-29T11:23:53Z | - |
dc.date.issued | 2019-09-27 | - |
dc.identifier.issn | 0031-1820 | - |
dc.identifier.uri | http://hdl.handle.net/2445/143332 | - |
dc.description.abstract | C-mannosylation was recently identified in the thrombospondin-related anonymous protein (TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum C-mannosyltransferase (Pf DPY-19) was demonstrated to modify thrombospondin type 1 repeat (TSR) domains in vitro, exhibiting a different acceptor specificity than their mammalian counterparts. According to the described minimal acceptor of Pf DPY19, several TSR domain-containing proteins of P. falciparum could be C-mannosylated in vivo. However, the relevance of this protein modification for the parasite viability remains unknown. In the present study, we used CRISPR/Cas9 technology to generate a Pf DPY19 null mutant, demonstrating that this glycosyltransferase is not essential for the asexual blood development of the parasite. Pf DPY19 gene disruption was not associated with a growth phenotype, not even under endoplasmic reticulum-stressing conditions that could impair protein folding. The data presented in this work strongly suggest that Pf DPY19 is unlikely to play a critical role in the asexual blood stages of the parasite, at least under in vitro conditions. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Cambridge University Press | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1017/S0031182019001380 | - |
dc.relation.ispartof | Parasitology, 2019 | - |
dc.relation.uri | http://dx.doi.org/10.1017/S0031182019001380 | - |
dc.rights | cc by (c) Cambridge University Press, 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | - |
dc.source | Articles publicats en revistes (ISGlobal) | - |
dc.subject.classification | Plasmodium falciparum | - |
dc.subject.classification | Paràsits | - |
dc.subject.other | Parasites | - |
dc.title | A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2019-10-25T18:00:30Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31559936 | - |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
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File | Description | Size | Format | |
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Lopez-GutierrezB_Parasitology_2019.pdf | 284.6 kB | Adobe PDF | View/Open |
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