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Title: | Passive stiffness of left ventricular myocardial tissue is reduced by ovariectomy in a post-menopause mouse model |
Author: | Farré, Núria Jorba, Ignasi Torres, Marta Falcones, Bryan Martí Almor, Julio Farré Ventura, Ramon Almendros López, Isaac Navajas Navarro, Daniel |
Keywords: | Hormones Insuficiència cardíaca Menopausa Models animals en la investigació Hormones Heart failure Menopause Animal models in research |
Issue Date: | 5-Nov-2018 |
Publisher: | Frontiers Media |
Abstract: | Background: Heart failure (HF) - a very prevalent disease with high morbidity and mortality - usually presents with diastolic dysfunction. Although post-menopause women are at increased risk of HF and diastolic dysfunction, poor attention has been paid to clinically and experimentally investigate this group of patients. Specifically, whether myocardial stiffness is affected by menopause is unknown. Aim: To investigate whether loss of female sexual hormones modifies the Young's modulus (E) of left ventricular (LV) myocardial tissue in a mouse model of menopause induced by ovariectomy (OVX). Methods: After 6 months of bilateral OVX, eight mice were sacrificed, fresh LV myocardial strips were prepared (∼8 × 1 × 1 mm), and their passive stress-stretch relationship was measured. E was computed by exponential fitting of the stress-stretch relationship. Subsequently, to assess the relative role of cellular and extracellular matrix components in determining OVX-induced changes in E, the tissues strips were decellularized and subjected to the same stretching protocol to measure E. A control group of eight sham-OVX mice was simultaneously studied. Results: E (kPa; m ± SE) in OVX mice was ∼twofold lower than in controls (11.7 ± 1.8 and 22.1 ± 4.4, respectively; p < 0.05). No significant difference between groups was found in E of the decellularized tissue (31.4 ± 12.05 and 40.9 ± 11.5, respectively; p = 0.58). Conclusion: Loss of female sexual hormones in an OVX model induces a reduction in the passive stiffness of myocardial tissue, suggesting that active relaxation should play a counterbalancing role in diastolic dysfunction in post-menopausal women with HF. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fphys.2018.01545 |
It is part of: | Frontiers in Physiology, 2018, vol. 9, p. 1545 |
URI: | http://hdl.handle.net/2445/146383 |
Related resource: | https://doi.org/10.3389/fphys.2018.01545 |
ISSN: | 1664-042X |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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