Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/148001
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mourglia-Ettlin, Gustavo | - |
dc.contributor.author | Miles, Sebastián | - |
dc.contributor.author | Velasco de Andrés, María | - |
dc.contributor.author | Armiger Borràs, Noelia | - |
dc.contributor.author | Cucher, Marcela | - |
dc.contributor.author | Dematteis, Sylvia | - |
dc.contributor.author | Lozano Soto, Francisco | - |
dc.date.accessioned | 2020-01-16T12:01:52Z | - |
dc.date.available | 2020-01-16T12:01:52Z | - |
dc.date.issued | 2018-11-30 | - |
dc.identifier.issn | 1935-2735 | - |
dc.identifier.uri | http://hdl.handle.net/2445/148001 | - |
dc.description.abstract | Background: Scavenger Receptors (SRs) from the host's innate immune system are known to bind multiple ligands to promote the removal of non-self or altered-self targets. CD5 and CD6 are two highly homologous class I SRs mainly expressed on all T cells and the B1a cell subset, and involved in the fine tuning of activation and differentiation signals delivered by the antigen-specific receptors (TCR and BCR, respectively), to which they physically associate. Additionally, CD5 and CD6 have been shown to interact with and sense the presence of conserved pathogen-associated structures from bacteria, fungi and/or viruses. Methodology/Principal findings: We report herein the interaction of CD5 and CD6 lymphocyte surface receptors with Echinococcus granulosus sensu lato (s.l.). Binding studies show that both soluble and membrane-bound forms of CD5 and CD6 bind to intact viable protoscoleces from E. granulosus s.l. through recognition of metaperiodate-resistant tegumental components. Proteomic analyses allowed identification of thioredoxin peroxidase for CD5, and peptidyl-prolyl cis-trans isomerase (cyclophilin) and endophilin B1 (antigen P-29) for CD6, as their potential interactors. Further in vitro assays demonstrate that membrane-bound or soluble CD5 and CD6 forms differentially modulate the pro- and anti-inflammatory cytokine release induced following peritoneal cells exposure to E. granulosus s.l. tegumental components. Importantly, prophylactic infusion of soluble CD5 or CD6 significantly ameliorated the infection outcome in the mouse model of secondary cystic echinococcosis. Conclusions/Significance Taken together, the results expand the pathogen binding properties of CD5 and CD6 and provide novel evidence for their therapeutic potential in human cystic echinococcosis. Author summary: Scavenger Receptors (SRs) are constituents of host's innate immune system able to sense and remove altered-self and/or pathogen components. Data on their interaction with helminth parasites is scarce. In this work, we describe that CD5 and CD6 -two lymphoid SRs previously reported to interact with conserved structures from bacteria, fungi and viruses- recognize tegumental components in the cestode parasite Echinococcus granulosus sensu lato (s.l.). Moreover, both receptors differentially modulate the cytokine release by host cells exposed to E. granulosus s.l. tegumental components. Importantly, the infusion of soluble forms of CD5 or CD6 improve infection outcomes in a murine model of secondary cystic echinococcosis. In summary, our results expand the pathogen binding properties of CD5 and CD6 and suggest their therapeutic potential against helminth infections. | - |
dc.format.extent | 24 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pntd.0006891 | - |
dc.relation.ispartof | PLoS Neglected Tropical Diseases, 2018, vol. 12, num. 11, p. e0006891 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pntd.0006891 | - |
dc.rights | cc-by (c) Mourglia-Ettlin, Gustavo et al., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Sistema immunitari | - |
dc.subject.classification | Cèl·lules T | - |
dc.subject.other | Immune system | - |
dc.subject.other | T cells | - |
dc.title | The ectodomains of the lymphocyte scavenger receptors CD5 and CD6 interact with tegumental antigens from Echinococcus granulosus sensu lato and protect mice against secondary cystic echinococcosis | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 692391 | - |
dc.date.updated | 2020-01-16T12:01:53Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30500820 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
692391.pdf | 2.4 MB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License