Please use this identifier to cite or link to this item:
Title: Rosetting in Plasmodium vivax: A Cytoadhesion Phenotype Associated with Anaemia
Author: Marín Menéndez, Alejandro
Bardají, Azucena
Martínez Espinosa, Flor E.
Bôtto Menezes, Camila
Lacerda, Marcus Vinícius Guimarães
Ortiz, Jon
Cisteró, Pau
Piqueras, Mireia
Felger, Ingrid
Mueller, Ivo
Ordi i Majà, Jaume
Portillo Obando, Hernando A. del
Menéndez, Clara
Wahlgren, Mats
Mayor Aparicio, Alfredo Gabriel
Keywords: Malalties parasitàries
Plasmodium vivax
Parasitic diseases
Plasmodium vivax
Issue Date: 4-Apr-2013
Publisher: Public Library of Science (PLoS)
Abstract: Background: Plasmodium vivax can potentially lead to life-threatening episodes but the mechanisms underlying severe disease remain poorly defined. Cytoadhesion of infected erythrocytes may contribute to P. vivax sequestration and organ injury although its physiological impact is still unknown. Here, we aimed to describe clinically-relevant cytoadhesive phenotypes of P. vivax isolates. Methodology/Principal findings: Rosetting and adhesion to CSA, CD36, ICAM1, placental and brain cryosections were determined in P. vivax peripheral isolates from 12 pregnant women, 24 non-pregnant women and 23 men from Manaus (Brazil). P. falciparum co-infection was excluded by PCR and P. vivax isolates were genotyped by assessing the size polymorphism of microsatellites ms2, ms20 and msp1F3 through capillary electrophoresis of PCR products. P. vivax monoinfection was confirmed by PCR in 59 isolates, with 50 (85%) of them being single-clone infections. One P. vivax haplotype was more frequently found among pregnant women (33%) than in non-pregnant women (0%) and men (4%; p = 0.010). Rosetting was observed in 64% of the isolates, adhesion to CSA in 15%, to ICAM1 in 12% and to placental cryosections in 9%, being similar among pregnant and non-pregnant groups. Intensity of rosetting was higher among anaemic individuals compared to non-anaemic (p = 0.010) and decreased with increasing haematocrit (p = 0.033) and haemoglobin levels (p = 0.015). Conclusions/Significance: P. vivax peripheral isolates from pregnant women do not exhibit a prominent adhesion to CSA, although other parasite phenotypes still unknown may increase the propagation of certain P. vivax clones observed among pregnant hosts. Rosetting is a frequent cytoadhesive phenotype in P. vivax infections that may contribute to the development of anaemia.
Note: Reproducció del document publicat a:
It is part of: PLoS Neglected Tropical Diseases, 2013, vol. 7, num. 4, p. e2155
Related resource:
ISSN: 1935-2735
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

Files in This Item:
File Description SizeFormat 
626138.pdf461.7 kBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons