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http://hdl.handle.net/2445/149617
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DC Field | Value | Language |
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dc.contributor.author | Villegas, Lorena | - |
dc.contributor.author | Peró Gascón, Roger | - |
dc.contributor.author | Benavente Moreno, Fernando J. (Julián) | - |
dc.contributor.author | Barbosa Torralbo, José | - |
dc.contributor.author | Sanz Nebot, María Victoria | - |
dc.date.accessioned | 2020-02-07T15:00:49Z | - |
dc.date.available | 2021-02-10T06:10:16Z | - |
dc.date.issued | 2019-02-10 | - |
dc.identifier.issn | 0039-9140 | - |
dc.identifier.uri | http://hdl.handle.net/2445/149617 | - |
dc.description.abstract | In this study, we present the use of microreactors packed with immobilized trypsin particles for the rapid and efficient bottom-up analysis of proteins by on-line immobilized enzyme microreactor capillary electrophoresis mass spectrometry (IMER-CE-MS). The results obtained digesting β-lactoglogulin (β-LG) off-line with free trypsin in solution and with immobilized trypsin particles were taken as a reference for the optimization of the on-line protein digestion. Under the optimized conditions, on-line digestion, separation and characterization of the protein digests were possible in less than 30 min. The limit of detection for complete sequence coverage was around 10 µg mL-1 (~500 µM) of β-LG, the repeatability was comparable to the off-line digestion methods and the microreactor could be reused until thirty times. The good performance of IMER-CE-MS was also demonstrated for several other proteins as α-casein (α-CSN), β-casein (β-CSN), and κ-casein (κ-CSN), as well as for a complex protein mixture (an Escherichia coli whole cell lysate). | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.talanta.2019.02.039 | - |
dc.relation.ispartof | Talanta, 2019, vol. 199, p. 116-123 | - |
dc.relation.uri | https://doi.org/10.1016/j.talanta.2019.02.039 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Electroforesi capil·lar | - |
dc.subject.classification | Enzims | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.other | Capillary electrophoresis | - |
dc.subject.other | Enzymes | - |
dc.subject.other | Mass spectrometry | - |
dc.title | On-line protein digestion by immobilized enzyme microreactor capillary electrophoresis-mass spectrometry | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 681292 | - |
dc.date.updated | 2020-02-07T15:00:49Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
File | Description | Size | Format | |
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681292.pdf | 1.6 MB | Adobe PDF | View/Open |
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