Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/149622
Title: | On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of blood α-synuclein |
Author: | Peró Gascón, Roger Benavente Moreno, Fernando J. (Julián) Minic, Zoran Berezovski, Maxim V. Sanz Nebot, María Victoria |
Keywords: | Electroforesi capil·lar Espectrometria de masses Alfa-sinucleïna Capillary electrophoresis Mass spectrometry Alpha-synuclein |
Issue Date: | 11-Dec-2019 |
Publisher: | American Chemical Society |
Abstract: | In this paper, an on-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry method is described for the purification, preconcentration, separation, and characterization of α-synuclein (α-syn) in blood at the intact protein level. A single-stranded DNA aptamer is used to bind with high affinity and selectivity α-syn, which is a major component of Lewy bodies, the typical aggregated protein deposits found in Parkinson's disease (PD). Under the conditions optimized with recombinant α-syn, repeatability (2.1 and 5.4% percent relative standard deviation for migration times and peak areas, respectively) and microcartridge lifetime (around 20 analyses/microcartridge) were good, the method was linear between 0.5 and 10 µg·mL-1 and limit of detection was 0.2 µg·mL-1 (100 times lower than by CE-MS, 20 µg·mL-1). The method was subsequently applied to the analysis of endogenous α-syn from red blood cells lysate of healthy controls and PD patients. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1021/acs.analchem.9b04802 |
It is part of: | Analytical Chemistry, 2019, vol. 92, p. 1525-1533 |
URI: | https://hdl.handle.net/2445/149622 |
Related resource: | https://doi.org/10.1021/acs.analchem.9b04802 |
ISSN: | 0003-2700 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
Files in This Item:
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695647.pdf | 1.18 MB | Adobe PDF | View/Open |
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