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https://hdl.handle.net/2445/151262
Título: | Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex |
Autor: | Benabou Zdaou, Sanae Eritja i Casadellà, Ramon Gargallo Gómez, Raimundo |
Materia: | Àcids nucleics G-estructures Oligonucleòtids Nucleic acids G-structures Oligonucleotides |
Fecha de publicación: | 2013 |
Publicado por: | Hindawi |
Resumen: | The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles. |
Nota: | Reproducció del document publicat a: https://doi.org/10.1155/2013/631875 |
Es parte de: | ISRN Biochemistry, 2013, vol. 2013, p. 1-7 |
URI: | https://hdl.handle.net/2445/151262 |
Recurso relacionado: | https://doi.org/10.1155/2013/631875 |
ISSN: | 2090-7729 |
Aparece en las colecciones: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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