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Title: Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
Author: Benabou Zdaou, Sanae
Eritja i Casadellà, Ramon
Gargallo Gómez, Raimundo
Keywords: Àcids nucleics
Nucleic acids
Issue Date: 2013
Publisher: Hindawi
Abstract: The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
Note: Reproducció del document publicat a:
It is part of: ISRN Biochemistry, 2013, vol. 2013, p. 1-7
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ISSN: 2090-7729
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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