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http://hdl.handle.net/2445/151827
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DC Field | Value | Language |
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dc.contributor.author | Duran, Bruno Oliveira da Silva | - |
dc.contributor.author | Dal-Pai-Silva, Maeli | - |
dc.contributor.author | García de la Serrana Castillo, Daniel | - |
dc.date.accessioned | 2020-03-03T15:20:22Z | - |
dc.date.available | 2020-12-23T06:10:17Z | - |
dc.date.issued | 2019-12-23 | - |
dc.identifier.issn | 0022-0949 | - |
dc.identifier.uri | http://hdl.handle.net/2445/151827 | - |
dc.description.abstract | Muscle fibres are classified as fast, intermediate and slow. In vitro myoblast cell culture model from fast muscle is a very useful tool to study muscle growth and development; however, similar models for slow muscle do not exist. Owing to the compartmentalization of fish muscle fibres, we have developed a slow myoblast cell culture for rainbow trout (Oncorhynchus mykiss). Slow and fast muscle-derived myoblasts have similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1α. We also characterized the mir-133 and mir-499 microRNA families in trout slow and fast myoblasts as a case study during myogenesis and in response to electrostimulation. Three mir-133 (a-1a, a-1b and a-2) and four mir-499 (aa, ab, ba and bb) paralogues were identified for rainbow trout and named base on their phylogenetic relationship to zebrafish and Atlantic salmon orthologues. Omy-mir-499ab and omy-mir-499bb had 0.6 and 0.5-fold higher expression in slow myoblasts compared with fast myoblasts, whereas mir-133 duplicates had similar levels in both phenotypes and little variation during development. Slow myoblasts also showed increased expression for omy-mir-499b paralogues in response to chronic electrostimulation (7-fold increase for omy-mir-499ba and 2.5-fold increase for omy-mir-499bb). The higher expression of mir-499 paralogues in slow myoblasts suggests a role in phenotype determination, while the lack of significant differences of mir-133 copies during culture development might indicate a different role in fish compared with mammals. We have also found signs of sub-functionalization of mir-499 paralogues after electrostimulation, with omy-mir-499b copies more responsive to electrical signals. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | The Company of Biologists | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1242/jeb.216390 | - |
dc.relation.ispartof | Journal of Experimental Biology, 2019, vol. 223, p. 216390 | - |
dc.relation.uri | https://doi.org/10.1242/jeb.216390 | - |
dc.rights | (c) The Company of Biologists, 2019 | - |
dc.source | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) | - |
dc.subject.classification | Esquelet humà | - |
dc.subject.classification | Fibres | - |
dc.subject.classification | Músculs | - |
dc.subject.other | Human skeleton | - |
dc.subject.other | Fibers | - |
dc.subject.other | Muscles | - |
dc.title | Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle and the characterization of mir-133 and mir-499 families as a case study | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 694842 | - |
dc.date.updated | 2020-03-03T15:20:23Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
Files in This Item:
File | Description | Size | Format | |
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694842.pdf | 1.65 MB | Adobe PDF | View/Open |
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