Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/155452
Title: | In vitro self-organized mouse small intestinal epithelial monolayer protocol |
Author: | Altay, Gizem Batlle, Eduard Fernández-Majada, Vanesa Martínez Fraiz, Elena |
Keywords: | Cèl·lules epitelials Ratolins (Animals de laboratori) Epithelial cells Mice as laboratory animals |
Issue Date: | 5-Feb-2020 |
Publisher: | Bio-protocol LLC |
Abstract: | Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing capacities. Here, we show a simple method to obtain mouse small intestine-derived epithelial monolayers organized into proliferative crypt-like domains, containing stem cells, and differentiated villus-like regions, closely resembling the in vivo cell composition and distribution. In addition, we adapted our model to a tissue culture format compatible with functional studies and prove close to physiological barrier properties of our in vitro epithelial monolayers. Thus, we have set-up a protocol to generate physiologically relevant intestinal epithelial monolayers to be employed in assays where independent access to both luminal and basolateral compartments is needed, such as drug absorption, intracellular trafficking and microbiome-epithelium interaction assays. |
It is part of: | Bio-protocol, 2020, vol. 10, num. 3, p. e3514 |
URI: | https://hdl.handle.net/2445/155452 |
Related resource: | https://doi.org/10.21769/BioProtoc.3514 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Bio-protocol3514.pdf | 2.65 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.