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Title: Polarized actin and VE-Cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis
Author: Cao, Jiahui
Ehling, Manuel
März, Sigrid
Seebach, Jochen
Tarbashevich, Katsiaryna
Sixta, Tomas
Pitulescu, Mara E.
Werner, Ann-Cathrin
Flach, Boris
Montañez, Eloi
Raz, Erez
Adams, Ralf H.
Schnittler, Hans
Keywords: Metabolisme
Antígens CD
Factor de creixement de l'endoteli vascular
CD antigens
Vascular endothelial growth factors
Issue Date: 20-Dec-2017
Publisher: Nature Publishing Group
Abstract: VEGFR-2/Notch signalling regulates angiogenesis in part by driving the remodelling of endothelial cell junctions and by inducing cell migration. Here, we show that VEGF-induced polarized cell elongation increases cell perimeter and decreases the relative VE-cadherin concentration at junctions, triggering polarized formation of actin-driven junction-associated intermittent lamellipodia (JAIL) under control of the WASP/WAVE/ARP2/3 complex. JAIL allow formation of new VE-cadherin adhesion sites that are critical for cell migration and monolayer integrity. Whereas at the leading edge of the cell, large JAIL drive cell migration with supportive contraction, lateral junctions show small JAIL that allow relative cell movement. VEGFR-2 activation initiates cell elongation through dephosphorylation of junctional myosin light chain II, which leads to a local loss of tension to induce JAIL-mediated junctional remodelling. These events require both microtubules and polarized Rac activity. Together, we propose a model where polarized JAIL formation drives directed cell migration and junctional remodelling during sprouting angiogenesis.
Note: Reproducció del document publicat a:
It is part of: Nature Communications, 2017, vol. 8, num. 1, p. 2210
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ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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