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http://hdl.handle.net/2445/156637
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DC Field | Value | Language |
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dc.contributor.author | Pulido Salgado, Marta | - |
dc.contributor.author | Vidal Taboada, José Manuel | - |
dc.contributor.author | García-Díaz Barriga, Gerardo | - |
dc.contributor.author | Solà i Subirana, Carme | - |
dc.contributor.author | Saura Martí, Josep | - |
dc.date.accessioned | 2020-04-21T22:45:30Z | - |
dc.date.available | 2020-04-21T22:45:30Z | - |
dc.date.issued | 2018-10-31 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/156637 | - |
dc.description.abstract | Microglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with LPS and LPS + IFNγ have not been properly compared. Here, we treated murine primary microglial cultures with LPS or LPS + IFNγ for 6 hours and then performed RNA-Sequencing. Gene expression patterns induced by the treatments were obtained by WGCNA and 11 different expression profiles were found, showing differential responses to LPS and LPS + IFNγ in many genes. Interestingly, a subset of genes involved in Parkinson's, Alzheimer's and Huntington's disease were downregulated by both treatments. By DESeq analysis we found differentially upregulated and downregulated genes that confirmed LPS and LPS + IFNγ as inducers of microglial pro-inflammatory responses, but also highlighted their involvement in specific cell functions. In response to LPS, microglia tended to be more proliferative, pro-inflammatory and phagocytic; whereas LPS + IFNγ inhibited genes were involved in pain, cell division and, unexpectedly, production of some inflammatory mediators. In summary, this study provides a detailed description of the transcriptome of LPS- and LPS + IFNγ treated primary microglial cultures. It may be useful to determine whether these in vitro phenotypes resemble microglia in in vivo pathological conditions. | - |
dc.format.extent | 21 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-018-34412-9 | - |
dc.relation.ispartof | Scientific Reports, 2018, vol. 8, p. 16906 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-018-34412-9 | - |
dc.rights | cc-by (c) Pulido Salgado, Marta et al., 2018 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Micròglia | - |
dc.subject.classification | Malalties del sistema nerviós central | - |
dc.subject.classification | RNA | - |
dc.subject.other | Microglia | - |
dc.subject.other | Central nervous system diseases | - |
dc.subject.other | RNA | - |
dc.title | RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 686512 | - |
dc.date.updated | 2020-04-21T22:45:30Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 30382133 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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686512.pdf | 3.88 MB | Adobe PDF | View/Open |
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