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Title: Functional characterization of a multi-cancer risk locus on chr5p15.33 reveals regulation of TERT by ZNF148
Author: Fang, Jun
Jia, Jinping
Makowski, Matthew
Xu, Mai
Wang, Zhaoming
Zhang, Tongwu
Hoskins, Jason W.
Carrera Álvarez, Cristina
PanScan Consortium
TRICL Consortium
GenoMEL Consortium
Keywords: Càncer de pulmó
Càncer de pell
Càncer de pàncrees
Lung cancer
Skin cancer
Pancreas cancer
Issue Date: 2-May-2017
Publisher: Nature Publishing Group
Abstract: Genome wide association studies (GWAS) have mapped multiple independent cancer susceptibility loci to chr5p15.33. Here, we show that fine-mapping of pancreatic and testicular cancer GWAS within one of these loci (Region 2 in CLPTM1L) focuses the signal to nine highly correlated SNPs. Of these, rs36115365-C associated with increased pancreatic and testicular but decreased lung cancer and melanoma risk, and exhibited preferred protein-binding and enhanced regulatory activity. Transcriptional gene silencing of this regulatory element repressed TERT expression in an allele-specific manner. Proteomic analysis identifies allele-preferred binding of Zinc finger protein 148 (ZNF148) to rs36115365-C, further supported by binding of purified recombinant ZNF148. Knockdown of ZNF148 results in reduced TERT expression, telomerase activity and telomere length. Our results indicate that the association with chr5p15.33-Region 2 may be explained by rs36115365, a variant influencing TERT expression via ZNF148 in a manner consistent with elevated TERT in carriers of the C allele.
Note: Reproducció del document publicat a:
It is part of: Nature Communications, 2018, vol. 8, p. 15034
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ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Medicina)
Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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