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Title: Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
Author: Balcells Nadal, Cristina
Pastor, Isabel
Pitulice, Laura
Hernández, Claudia
Via Nadal, Mireia
Garcés, Josep Lluís
Madurga Díez, Sergio
Vilaseca i Font, Eudald
Isvoran, Adriana
Cascante i Serratosa, Marta
Mas i Pujadas, Francesc
Keywords: Cinètica enzimàtica
Enzyme kinetics
Issue Date: Jul-2015
Publisher: Universitatea de Vest din Timişoara
Abstract: In the present work, the volume exclusion phenomenon, also known as macromolecular crowding, has been applied to the field of enzyme kinetics. It has been approached by adding polymeric obstacles in the media of different enzymatic reactions. The concentration and size of these obstacles have been changed systematically in order to obtain kinetic information about each reaction. Results indicate that the performance of a certain enzyme always depends on the amount of excluded volume. However, only large, oligomeric proteins display an obstacle size-dependent behavior. Besides, crowding can hinder diffusion to the extent of being capable of shifting reaction control from activation to diffusion.
Note: Reproducció del document publicat a:
It is part of: New Frontiers in Chemistry, 2015, vol. 24, num. 1, p. 3-16
ISSN: 2393-2171
Appears in Collections:Articles publicats en revistes (Ciència dels Materials i Química Física)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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