Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/158997
Title: Thermoreversible Gel-Loaded Amphotericin B for the Treatment of Dermal and Vaginal Candidiasis
Author: Sosa Díaz, Lilian Elisa
Calpena Campmany, Ana Cristina
Silva Abreu, Marcelle
Espinoza, Lupe Carolina
Rincón, María
Bozal de Febrer, Núria
Domènech Cabrera, Òscar
Rodríguez Lagunas, María José
Clares Naveros, Beatriz
Keywords: Candidiasi
Copolímers
Gels (Farmàcia)
Candidiasis
Copolymers
Gels (Pharmacy)
Issue Date: 3-Jul-2019
Publisher: MDPI
Abstract: The present study was designed to develop a thermoreversible gel of Pluronic (P407) loaded amphotericin B (AmB-gel) for the dermal and vaginal treatment of candidiasis. P407 was used as a copolymer to exploit potential advantages related to increasing drug concentration in the tissue layer in order to provide a local effect. Parameters including internal structure, swelling, porosity, and short-term stability were determined. In addition, drug release profile and ex vivo skin and vaginal permeation studies were carried out. Antifungal efficacy was evaluated against strains of Candida spp. and atomic force microscopy (AFM) supported the results. The tolerance of AmB-gel was studied by evaluating biomechanical properties of skin and determining the irritation level in scarified rabbit skin supported by histological analysis. Results confirmed the development of a thermoreversible AmB-gel with high porosity exhibiting Newtonian behavior at 4 °C and pseudoplasticity at 32 °C as well as optimal stability for at least 90 days. The Amb-gel provided a sustained drug release following a Boltzmann sigmoidal model. Non permeation was observed in skin and vaginal mucosa, showing a high retained amount of AmB of 960.0 and 737.3 µg/g/cm2, respectively. In vitro antifungal efficacy showed that AmB-gel was more effective than Free-AmB in inhibiting strains of Candida spp. and these results were corroborated by AFM. Finally, tolerance studies showed that its application did not induce skin irritation nor alter its biophysical properties. Together, these results confirmed that AmB-gel could be proposed as a promising candidate for the clinical status in the treatment of skin and vaginal candidiasis.
Note: Reproducció del document publicat a: https://doi.org/10.3390/pharmaceutics11070312
It is part of: Pharmaceutics, 2019, vol. 11, num. 7, p. 312
URI: https://hdl.handle.net/2445/158997
Related resource: https://doi.org/10.3390/pharmaceutics11070312
ISSN: 1999-4923
Appears in Collections:Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Bioquímica i Fisiologia)

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