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Title: Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality
Author: Valls Margarit, Maria
Iglesias García, Olalla
Guglielmo, Claudia Di
Sarlabous, Leonardo
Tadevosyan, Karine
Paoli, Roberto
Comelles Pujadas, Jordi
Blanco Almazán, Dolores
Jiménez Delgado, Senda
Castillo Fernández, Óscar
Samitier i Martí, Josep
Jané, Raimon
Martínez Fraiz, Elena
Raya Chamorro, Ángel
Keywords: Fisiologia humana
Human physiology
Issue Date: 9-Jul-2019
Publisher: Elsevier
Abstract: In vitro surrogate models of human cardiac tissue hold great promise in disease modeling, cardiotoxicity testing, and future applications in regenerative medicine. However, the generation of engineered human cardiac constructs with tissue-like functionality is currently thwarted by difficulties in achieving efficient maturation at the cellular and/or tissular level. Here, we report on the design and implementation of a platform for the production of engineered cardiac macrotissues from human pluripotent stem cells (PSCs), which we term "CardioSlice." PSC-derived cardiomyocytes, together with human fibroblasts, are seeded into large 3D porous scaffolds and cultured using a parallelized perfusion bioreactor with custom-made culture chambers. Continuous electrical stimulation for 2 weeks promotes cardiomyocyte alignment and synchronization, and the emergence of cardiac tissue-like properties. These include electrocardiogram-like signals that can be readily measured on the surface of CardioSlice constructs, and a response to proarrhythmic drugs that is predictive of their effect in human patients.
Note: Reproducció del document publicat a:
It is part of: Stem Cell Reports, 2019, vol. 13, num. 1, p. 207-220
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ISSN: 2213-6711
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)

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