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Title: Development and Validation of a New High-Performance Liquid Chromatography Method for the Simultaneous Quantification of Coenzyme Q10, Phosphatidylserine, and Vitamin C from a Cutting-Edge Liposomal Vehiculization
Author: Ruiz García, Marta
Pérez Lozano, Pilar
Mercadé Frutos, Débora
Nardi Ricart, Anna
Suñé Pou, Marc
Cano Sarabia, Mary
García Jimeno, Sonia
Suñé i Negre, Josep M. (Josep Maria)
Maspoch, Daniel
García Montoya, Encarna
Keywords: Cromatografia de líquids
Liquid chromatography
Issue Date: 26-Nov-2019
Publisher: American Chemical Society
Abstract: A high-performance liquid chromatography (HPLC) method was developed to simultaneously quantify three widely used active substances such as coenzyme Q10, phosphatidylserine, and vitamin C. This new method optimizes current timing and costs in the analyses of these three active substances. Additionally, since the analyzed compounds were encapsulated on a cutting-edge liposomal formulation, further processing was necessary to be developed prior to HPLC analyses. The technique was studied and adequately validated in accordance with the guidelines of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) regarding selectivity, linearity, accuracy, precision, and robustness. After data treatment of results, linear regressions for all active substances showed an optimal linearity with a correlation coefficient of >0.999 in the concentration range between 70 to 130% of the liposomal formulation and less than a 3% relative standard deviation (RSD) in accuracy and precision.
Note: Reproducció del document publicat a:
It is part of: ACS Omega , 2019, vol. 4, num. 22, p. 19710-19715
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ISSN: 2470-1343
Appears in Collections:Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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