Please use this identifier to cite or link to this item:
Title: Short-term responses to fatty acids on lipid metabolism and adipogenesis in rainbow trout (Oncorhynchus mykiss)
Author: Riera Heredia, Natàlia
Lutfi Royo, Esmail
Sánchez-Moya, Albert
Gutiérrez Fruitós, Joaquín
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
Keywords: Truites (Peixos)
Teixit adipós
Adipose tissues
Issue Date: 27-Feb-2020
Publisher: MDPI
Abstract: Fish are rich in n-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Due to the increasing use of vegetable oils (VO), their proportion in diets has lowered, affecting lipid metabolism and fillet composition. Rainbow trout cultured preadipocytes were treated with representative FA found in fish oils (EPA and DHA) or VO (linoleic, LA and alpha-linolenic, ALA acids), while EPA and LA were also orally administered, to evaluate their effects on adipogenesis and lipid metabolism. In vitro, all FA increased lipid internalization, with ALA producing the highest effect, together with upregulating the FA transporter fatp1. In vivo, EPA or LA increased peroxisome proliferator-activated receptors ppara and pparb transcripts abundance in adipose tissue, suggesting elevated β-oxidation, contrary to the results obtained in liver. Furthermore, the increased expression of FA synthase (fas) and the FA translocase/cluster of differentiation (cd36) in adipose tissue indicated an enhanced uptake of lipids and lipogenesis de novo, whereas stable or low hepatic expression of genes involved in lipid transport and turnover was found. Thus, fish showed a similar tissue metabolic response to the short-term availability of EPA or LA in vivo, while in vitro VO-derived FA demonstrated greater potential inducing fat accumulation.
Note: Reproducció del document publicat a:
It is part of: International Journal of Molecular Sciences, 2020, vol. 21, p. 1623
Related resource:
ISSN: 1661-6596
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

Files in This Item:
File Description SizeFormat 
700196.pdf7.11 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons