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http://hdl.handle.net/2445/162038
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DC Field | Value | Language |
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dc.contributor.author | Peró Gascón, Roger | - |
dc.contributor.author | Giménez López, Estela | - |
dc.contributor.author | Sanz Nebot, María Victoria | - |
dc.contributor.author | Benavente Moreno, Fernando J. (Julián) | - |
dc.date.accessioned | 2020-05-22T11:21:57Z | - |
dc.date.available | 2022-05-12T05:10:19Z | - |
dc.date.issued | 2020-05-12 | - |
dc.identifier.issn | 0026-265X | - |
dc.identifier.uri | http://hdl.handle.net/2445/162038 | - |
dc.description.abstract | The analysis and detection of targeted peptides has received due attention in many fields of proteomics research, such as discovery of biomarkers. In this regard, capillary electrophoresis-mass spectrometry (CE-MS) is widely used, however, direct analysis of low-abundance peptides such as histidine-containing peptides (His-peptides) in complex matrixes by CE-MS remains an analytical challenge. In the present study, an immobilised metal affinity solid-phase extraction containing Ni(II) coupled on-line to capillary electrophoresis-mass spectrometry (IMA-SPE-CE-MS) method has been developed to selectively enrich His-peptides from protein tryptic digests and enhance sensitivity. The method was optimised with α-casein and validated with other standard proteins (β-casein and κ-casein). Later, it was applied to an Escherichia Coli (E. Coli) whole cell lysate. IMA-SPE-CE-MS was very selective and allowed an enrichment factor up to 100-fold. The on-line enrichment and separation method coupled to MS detection is straightforward and advantageous over off-line pretreatment methods in terms of simplicity, cost-effectiveness and throughput. | - |
dc.format.extent | 32 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.microc.2020.105013 | - |
dc.relation.ispartof | Microchemical Journal, 2020, vol. 267, p. 121881 | - |
dc.relation.uri | https://doi.org/10.1016/j.microc.2020.105013 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Electroforesi capil·lar | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.classification | Pèptids | - |
dc.subject.other | Capillary electrophoresis | - |
dc.subject.other | Mass spectrometry | - |
dc.subject.other | Peptides | - |
dc.title | Enrichment of histidine containing peptides by on-line immobilized metal affinity solid-phase extraction capillary electrophoresis-mass spectrometry | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 691981 | - |
dc.date.updated | 2020-05-22T11:21:58Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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File | Description | Size | Format | |
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691981.pdf | 853.04 kB | Adobe PDF | View/Open |
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