Please use this identifier to cite or link to this item:
Title: Glucose Restriction Promotes Osteocyte Specification by Activating a PGC-1α-Dependent Transcriptional Program.
Author: Sánchez de Diego, Cristina
Artigas, Natalia
Pimenta-Lopes, Carolina
Valer, José Antonio
Torrejón-Escribano, Benjamín
Gama-Perez, Pau
Villena, Josep A.
García-Roves, Pablo M. (Pablo Miguel)
Rosa López, José Luis
Ventura Pujol, Francesc
Keywords: Cèl·lules
Malalties dels ossos
ADN mitocondrial
Bone diseases
Mitochondrial DNA
Issue Date: 31-May-2019
Publisher: Elsevier
Abstract: Osteocytes, the most abundant of bone cells, differentiate while they remain buried within the bonematrix. This encasement limits their access to nutrients and likely affects their differentiation, a pro-cess that remains poorly defined. Here, we show that restriction in glucose supply promotes the oste-ocyte transcriptional program while also being associated with increased mitochondrial DNA levels.Glucose deprivation triggered the activation of the AMPK/PGC-1 pathway. AMPK and SIRT1 activa-tors or PGC-1aoverexpression are sufficient to enhance osteocyte gene expression in IDG-SW3 cells,murine primary osteoblasts, osteocytes, and organotypic/ex vivobone cultures. Conversely, osteo-blasts and osteocytes deficient inPpargc1aandbwere refractory to the effects of glucose restriction.Finally, conditional ablation of both genes in osteoblasts and osteocytes generate osteopenia andreduce osteocytic gene expression in mice. Altogether, we uncovered a role for PGC-1 in the regula-tion of osteocyte gene expression.
Note: Reproducció del document publicat a:
It is part of: iScience, 2019, vol. 15, num. 31, p. 79-94
Related resource:
ISSN: 2589-0042
Appears in Collections:Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Ciències Fisiològiques)

Files in This Item:
File Description SizeFormat 
690043.pdf9.48 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons