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Title: GATA2 Promotes Hematopoietic Development and Represses Cardiac Differentiation of Human Mesoderm
Author: Castaño, Julio
Aranda, Sergi
Bueno, Clara
Calero-Nieto, Fernando J.
Mejia-Ramirez, Eva
Mosquera Mayo, José Luís
Blanco, Enrique
Wang, Xiaonan
Prieto Rios, Cristina
Zabaleta, Lorea
Mereu, Elisabetta
Rovira, Meritxell
Jiménez-Delgado, Senda
Matson, Daniel R.
Heyn, Holger
Bresnick, Emery H.
Göttgens, Berthold
Di Croce, Luciano
Menéndez Buján, Pablo
Raya Chamorro, Ángel
Giorgetti, Alessandra
Keywords: Vertebrats
Embriologia humana
Human embryology
Issue Date: 10-Sep-2019
Publisher: Elsevier
Abstract: In vertebrates, GATA2 is a master regulator of hematopoiesis and is expressed throughout embryo development and in adult life. Although the essential role of GATA2 in mouse hematopoiesis is well established, its involvement during early human hematopoietic development is not clear. By combining time-controlled overexpression of GATA2 with genetic knockout experiments, we found that GATA2, at the mesoderm specification stage, promotes the generation of hemogenic endothelial progenitors and their further differentiation to hematopoietic progenitor cells, and negatively regulates cardiac differentiation. Surprisingly, genome-wide transcriptional and chromatin immunoprecipitation analysis showed that GATA2 bound to regulatory regions, and repressed the expression of cardiac development-related genes. Moreover, genes important for hematopoietic differentiation were upregulated by GATA2 in a mostly indirect manner. Collectively, our data reveal a hitherto unrecognized role of GATA2 as a repressor of cardiac fates, and highlight the importance of coordinating the specification and repression of alternative cell fates.
Note: Reproducció del document publicat a:
It is part of: Stem Cell Reports, 2019, vol. 13, num. 3, p. 515-529
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ISSN: 2213-6711
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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