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http://hdl.handle.net/2445/162068
Title: | GATA2 Promotes Hematopoietic Development and Represses Cardiac Differentiation of Human Mesoderm |
Author: | Castaño, Julio Aranda, Sergi Bueno, Clara Calero-Nieto, Fernando J. Mejia-Ramirez, Eva Mosquera Mayo, José Luís Blanco, Enrique Wang, Xiaonan Prieto Rios, Cristina Zabaleta, Lorea Mereu, Elisabetta Rovira, Meritxell Jiménez-Delgado, Senda Matson, Daniel R. Heyn, Holger Bresnick, Emery H. Göttgens, Berthold Di Croce, Luciano Menéndez Buján, Pablo Raya Chamorro, Ángel Giorgetti, Alessandra |
Keywords: | Vertebrats Hematopoesi Embriologia humana Cromatina Vertebrates Hematopoiesis Human embryology Chromatin |
Issue Date: | 10-Sep-2019 |
Publisher: | Elsevier |
Abstract: | In vertebrates, GATA2 is a master regulator of hematopoiesis and is expressed throughout embryo development and in adult life. Although the essential role of GATA2 in mouse hematopoiesis is well established, its involvement during early human hematopoietic development is not clear. By combining time-controlled overexpression of GATA2 with genetic knockout experiments, we found that GATA2, at the mesoderm specification stage, promotes the generation of hemogenic endothelial progenitors and their further differentiation to hematopoietic progenitor cells, and negatively regulates cardiac differentiation. Surprisingly, genome-wide transcriptional and chromatin immunoprecipitation analysis showed that GATA2 bound to regulatory regions, and repressed the expression of cardiac development-related genes. Moreover, genes important for hematopoietic differentiation were upregulated by GATA2 in a mostly indirect manner. Collectively, our data reveal a hitherto unrecognized role of GATA2 as a repressor of cardiac fates, and highlight the importance of coordinating the specification and repression of alternative cell fates. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2019.07.009 |
It is part of: | Stem Cell Reports, 2019, vol. 13, num. 3, p. 515-529 |
URI: | http://hdl.handle.net/2445/162068 |
Related resource: | https://doi.org/10.1016/j.stemcr.2019.07.009 |
ISSN: | 2213-6711 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
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