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http://hdl.handle.net/2445/162210
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DC Field | Value | Language |
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dc.contributor.author | Pont Villanueva, Laura | - |
dc.contributor.author | Marin, Gemma | - |
dc.contributor.author | Vergara-Barberán, Maria | - |
dc.contributor.author | Gagliardi, Leonardo G. | - |
dc.contributor.author | Sanz Nebot, María Victoria | - |
dc.contributor.author | Herrero-Martínez, J. M. | - |
dc.contributor.author | Benavente Moreno, Fernando J. (Julián) | - |
dc.date.accessioned | 2020-05-25T09:06:59Z | - |
dc.date.available | 2022-04-18T05:10:17Z | - |
dc.date.issued | 2020-04-18 | - |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | http://hdl.handle.net/2445/162210 | - |
dc.description.abstract | In this study, polymeric monoliths with gold nanoparticles (AuNP@monolith) were investigated as microcartridges for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS). "Plug-and-play" microcartridges (7 mm) were prepared from a glycidyl methacrylate (GMA)-based monolithic capillary column (5 cm x 250 µm i.d.), which was modified with ammonia and subsequently functionalized with gold nanoparticles (AuNPs). The performance of these novel microcartridges was evaluated with human transthyretin (TTR), which is a protein related to different types of familial amyloidotic polyneuropathies (FAP). Protein retention depended on the isoelectric point of the protein (TTR pI~5.4) and elution was achieved with a basic phosphate solution. Under the optimized conditions, limits of detection (LODs) for TTR by AuNP@monolith-SPE-CE-MS were 50 times lower than by CE-MS (5 vs 250 mg L−1, with an ion trap (IT) mass spectrometer). The sensitivity enhancement was similar compared to SPE-CE-MS using immunoaffinity (IA) microcartridges with intact antibodies against TTR. Linearity, repeatability in migration times and peak areas, reusability, reproducibility and application to serum samples were also evaluated | - |
dc.format.extent | 34 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2020.461097 | - |
dc.relation.ispartof | Journal of Chromatography A, 2020, vol. 1622, p. 461097-461097 | - |
dc.relation.uri | https://doi.org/10.1016/j.chroma.2020.461097 | - |
dc.rights | cc-by-nc-nd (c) Elsevier B.V., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Química i Química Analítica) | - |
dc.subject.classification | Electroforesi capil·lar | - |
dc.subject.classification | Or | - |
dc.subject.classification | Nanopartícules | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.other | Capillary electrophoresis | - |
dc.subject.other | Gold | - |
dc.subject.other | Nanoparticles | - |
dc.subject.other | Mass spectrometry | - |
dc.title | Polymeric monolithic microcartridges with gold nanoparticles for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 700994 | - |
dc.date.updated | 2020-05-25T09:06:59Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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700994.pdf | 621.73 kB | Adobe PDF | View/Open |
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