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Title: | Imbalance in the expression of genes associated with purinergic signalling in the lung and systemic arteries of COPDpatients. |
Author: | Careta, Oriol Cuevas, Ester Muñoz Esquerre, Mariana López Sánchez, Marta Pascual-González, Yuliana Dorca i Sargatal, Jordi Aliagas Marín, Elisabeth Santos Pérez, Salud |
Keywords: | Etiologia Malalties pulmonars obstructives cròniques Reacció en cadena de la polimerasa Etiology Chronic obstructive pulmonary diseases Polymerase chain reaction |
Issue Date: | 26-Feb-2019 |
Publisher: | Nature Publishing Group |
Abstract: | Growing evidence indicates that purinergic signalling is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD) and in the vascular remodelling that occurs in other disorders; however, its role in initial vascular changes of COPD is not entirely known. We hypothesised that expression of genes regulating extracellular ATP and adenosine levels would be altered in the lung and systemic arteries of COPD patients. Quantitative real-time PCR was performed to analyse the relative expression of 17 genes associated with purinergic signalling and inflammation in lungs and intercostal arteries of never smokers (NS) (n = 16), non-obstructed smokers (NOS) (n = 17) and COPD patients (n = 21). Gene expression of ATP-degrading enzymes was decreased in both tissues of NOS and COPD patients compared to NS. NT5E expression (gene transcribing for an AMP hydrolyzing ectonucleotidase) was increased in both tissues in NOS compared to the other groups. P1 and P2 receptors did not show changes in expression. Expression of genes associated with inflammation (interleukin-13) was upregulated only in lung tissues of COPD. These findings suggest that the expression of different extracellular ATP-degrading enzymes is altered in smokers (NOS and COPD patients), promoting inflammation. However, the high NT5E expression found only in NOS could compensate this inflammatory environment. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-019-39233-y |
It is part of: | Scientific Reports, 2019, vol. 9, p. 2796 |
URI: | http://hdl.handle.net/2445/162520 |
Related resource: | https://doi.org/10.1038/s41598-019-39233-y |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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