Please use this identifier to cite or link to this item:
Title: Imbalance in the expression of genes associated with purinergic signalling in the lung and systemic arteries of COPDpatients.
Author: Careta, Oriol
Cuevas, Ester
Muñoz Esquerre, Mariana
López Sánchez, Marta
Pascual-González, Yuliana
Dorca i Sargatal, Jordi
Aliagas Marín, Elisabeth
Santos Pérez, Salud
Keywords: Etiologia
Malalties pulmonars obstructives cròniques
Reacció en cadena de la polimerasa
Chronic obstructive pulmonary diseases
Polymerase chain reaction
Issue Date: 26-Feb-2019
Publisher: Nature Publishing Group
Abstract: Growing evidence indicates that purinergic signalling is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD) and in the vascular remodelling that occurs in other disorders; however, its role in initial vascular changes of COPD is not entirely known. We hypothesised that expression of genes regulating extracellular ATP and adenosine levels would be altered in the lung and systemic arteries of COPD patients. Quantitative real-time PCR was performed to analyse the relative expression of 17 genes associated with purinergic signalling and inflammation in lungs and intercostal arteries of never smokers (NS) (n = 16), non-obstructed smokers (NOS) (n = 17) and COPD patients (n = 21). Gene expression of ATP-degrading enzymes was decreased in both tissues of NOS and COPD patients compared to NS. NT5E expression (gene transcribing for an AMP hydrolyzing ectonucleotidase) was increased in both tissues in NOS compared to the other groups. P1 and P2 receptors did not show changes in expression. Expression of genes associated with inflammation (interleukin-13) was upregulated only in lung tissues of COPD. These findings suggest that the expression of different extracellular ATP-degrading enzymes is altered in smokers (NOS and COPD patients), promoting inflammation. However, the high NT5E expression found only in NOS could compensate this inflammatory environment.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2019, vol. 9, p. 2796
Related resource:
ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Files in This Item:
File Description SizeFormat 
684371.pdf1.82 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons