Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/162700
Title: | Relationship between Vancomycin MIC and Virulence Gene Expression in Clonal Complexes of Methicillin-Susceptible Staphylococcus aureus Strains Isolated from Left-sided Endocarditis |
Author: | Pericàs, Juan M. Cervera, Carlos García de la Mària, Cristina Sharma-Kuinkel, Batu K. Gonzales, Rachelle Moreno Camacho, Ma. Asunción Almela, M. (Manel) Falces Salvador, Carles Quintana, Eduard Fuster Pelfort, David Marco Reverté, Francesc Bayer, Arnold S. Fowler, Vance G. Miró Meda, José M. Hospital Clinic Endocarditis Study Group |
Keywords: | Endocarditis Infeccions per estafilococs Antibiòtics Endocarditis Staphylococcal infections Antibiotics |
Issue Date: | 21-Feb-2020 |
Publisher: | American Society for Microbiology |
Abstract: | Higher vancomycin MICs have been associated with more complicated courses and higher mortality rates in patients with Staphylococcus aureus bacteremia and infective endocarditis (IE). The aim of this study was to investigate whether the strains belonging to the cohort of 93 patients from a previously published study in which patients with strains with vancomycin MICs of ≥1.5 μg/ml presented higher mortality rates and systemic emboli than patients with strains with vancomycin MICs of <1.5 μg/ml had specific patterns of virulence factors, clonal complex (CC) types, or the ability to form biofilms. Vancomycin MICs were determined by Etest, and the isolates underwent spa typing to infer the CC, biofilm studies, a thrombin-induced platelet microbicidal assay, and multiplex PCR for the presence of virulence genes. We found no differences in genes encoding adhesins, toxins, or other putative virulence genes according to the vancomycin MIC group. CC30, CC34, and CC45 represented nearly half of the isolates, and there was no association with the vancomycin MIC. agr subgroups I and III predominated, with no association with the vancomycin MIC. Isolates with higher vancomycin MICs exhibited a poorer ability to form biofilms with and without the presence of vancomycin (2.03 versus 2.48 [P < 0.001], respectively, for isolates with higher vancomycin MICs and 2.60 versus 2.87 [P = 0.022], respectively, for isolates with lower vancomycin MICs). In the multivariable analysis, efb and V8 were risk factors for major emboli (adjusted odds ratio [aOR] = 7.5 and 95% confidence interval [CI] = 1.2 to 46.6 for efb, and aOR = 3.9 and 95% CI = 1.1 to 14.1 for V8), whereas no genotypic predictors of in-hospital mortality were found. No clear associations between genes encoding virulence factors, agr type, clonal complexes, mortality, and major embolic events according to vancomycin MIC group were found. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1128/AAC.01579-19 |
It is part of: | Antimicrobial Agents and Chemotherapy, 2020, vol. 64, num. 3, p. e01579-19 |
URI: | http://hdl.handle.net/2445/162700 |
Related resource: | https://doi.org/10.1128/AAC.01579-19 |
ISSN: | 0066-4804 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
695888.pdf | 336.19 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.