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Title: Distinct dual C-Cl isotope fractionation patterns during anaerobic biodegradation of 1,2-dichloroethane: potential to characterize microbial degradation in the field
Author: Palau, Jordi
Yu, R.
Hatijah Mortan, C.
Shouakar-Stash, O.
Rosell, Mònica
Freedman, D.
Sbarbati, C.
Fiorenza, S.
Aravena, R.
Marco-Urrea, E.
Elsner, M.
Soler i Gil, Albert
Hunkeler, D.
Keywords: Degradació dels sòls
Soil degradation
Issue Date: 14-Feb-2017
Publisher: American Chemical Society
Abstract: This study investigates, for the first time, dual C-Cl isotope fractionation during anaerobic biodegradation of 1,2-dichloroethane (1,2-DCA) via dihaloelimination by Dehalococcoides and Dehalogenimonas-containing enrichment cultures. Isotopic fractionation of 1,2-DCA (εbulkC and εbulkCl) for Dehalococcoides (−33.0 ± 0.4¿ and −5.1 ± 0.1¿) and Dehalogenimonas-containing microcosms (−23 ± 2¿ and −12.0 ± 0.8¿) resulted in distinctly different dual element C-Cl isotope correlations (Λ = Δδ13C/Δδ37Cl ≈ εbulkC/εbulkCl), 6.8 ± 0.2 and 1.89 ± 0.02, respectively. Determined isotope effects and detected products suggest that the difference on the obtained Λ values for biodihaloelimination could be associated with a different mode of concerted bond cleavage rather than two different reaction pathways (i.e., stepwise vs concerted). Λ values of 1,2-DCA were, for the first time, determined in two field sites under reducing conditions (2.1 ± 0.1 and 2.2 ± 2.9). They were similar to the one obtained for the Dehalogenimonas-containing microcosms (1.89 ± 0.02) and very different from those reported for aerobic degradation pathways in a previous laboratory study (7.6 ± 0.1 and 0.78 ± 0.03). Thus, this study illustrates the potential of a dual isotope analysis to differentiate between aerobic and anaerobic biodegradation pathways of 1,2-DCA in the field and suggests that this approach might also be used to characterize dihaloelimination of 1,2-DCA by different bacteria, which needs to be confirmed in future studies.
Note: Versió postprint del document publicat a:
It is part of: Environmental Science & Technology, 2017, vol. 51, num. 5, p. 2685- 2694
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ISSN: 0013-936X
Appears in Collections:Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)

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