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http://hdl.handle.net/2445/164344
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DC Field | Value | Language |
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dc.contributor.author | Sánchez Martín, Ma. Jesús | - |
dc.contributor.author | Busquets i Viñas, Ma. Antonia | - |
dc.contributor.author | Girona i Brumós, Victòria | - |
dc.contributor.author | Haro, Isabel | - |
dc.contributor.author | Alsina Esteller, Ma. Asunción | - |
dc.contributor.author | Pujol Cubells, Montserrat | - |
dc.date.accessioned | 2020-06-04T15:48:21Z | - |
dc.date.available | 2020-06-04T15:48:21Z | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 0005-2736 | - |
dc.identifier.uri | http://hdl.handle.net/2445/164344 | - |
dc.description.abstract | One way to gain information about the fusogenic potential of virus-derived synthetic peptides is to examine their interfacial properties and subsequently to study them in monolayers and bilayers. Here, we characterize the physicochemical surface properties of the peptide E1(64-81), whose sequence is AQLVGELGSLYGPLSVSA. This peptide is derived from the E1 structural protein of GBV-C/HGV which was previously shown to inhibit leakage of vesicular contents caused by the HIV-1 fusion peptide (HIV-1 FP). Mixed isotherms of E1(64-81) and HIV-1 FP were obtained and their Brewster angle microscopy (BAM) and atomic force microscopy (AFM) images showed that the peptide mixture forms a different structure that is not present in the pure peptide images. Studies with lipid monolayers (1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (DMPG) and 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG)) show that both peptides interact with all the lipids assayed but the effect that HIV-1 FP has on the monolayers is reduced in the presence of E1(64-81). Moreover, differential scanning calorimetry (DSC) experiments show the capacity of HIV-1 FP to modify the properties of the bilayer structure and the capacity of E1(64-81) to inhibit these modifications. Our results indicate that E1(64-81) interacts with HIV-1 FP to form a new structure, and that this may be the cause of the previously observed inhibition of the activity of HIV-1 FP by E1(64-81). | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.bbamem.2011.05.020 | - |
dc.relation.ispartof | Biochimica et Biophysica Acta-Biomembranes, 2011, vol. 1808, num. 9, p. 2178-2188 | - |
dc.relation.uri | https://doi.org/10.1016/j.bbamem.2011.05.020 | - |
dc.rights | (c) Elsevier B.V., 2011 | - |
dc.source | Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica) | - |
dc.subject.classification | Síntesi de pèptids | - |
dc.subject.classification | Hepatitis G | - |
dc.subject.classification | Virus GB C | - |
dc.subject.classification | VIH (Virus) | - |
dc.subject.other | Peptide synthesis | - |
dc.subject.other | Hepatitis G | - |
dc.subject.other | GB virus C | - |
dc.subject.other | HIV (Viruses) | - |
dc.title | Effect of E1(64-81) hepatitis G peptide on the in vitro interaction of HIV-1 Fusion Peptide with membrane models | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 595756 | - |
dc.date.updated | 2020-06-04T15:48:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica) Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB)) |
Files in This Item:
File | Description | Size | Format | |
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595756.pdf | 2.09 MB | Adobe PDF | View/Open |
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